318 NON-SACCHAROMYCETES OR DOUBTFUL YEASTS 



cells with a diameter of 10-25 /* are formed along with long budding 

 cells, incompletely formed and irregular. In liquid media the yeast 

 forms a thin pellicle on the surface and a deposit in the bottom of 

 the flask. On solid substrates, it forms small dots of growth about 

 0.5 to 1/z in diameter. Later these run together forming a shiny 

 mass almost mucous. The giant colonies on potato present a wrinkled 

 appearance. On agar and gelatin, in streaks and stabs, the vegetation 

 is at first with an even edge which after a certain time becomes 

 furrowed. Torula glutinis does not possess a very characteristic ap- 

 pearance and a series of related yeasts have been described under 

 this name. 



CRYPTOCOCCUS BAINIERI. Sartory 



This yeast was found by Bainier on the stems and leaves of the 

 nettle where it lived as a saprophyte. It has been described by Sar- 

 tory. 1 It is easily cultivated on all solid media (gelatin, agar, potato, 

 both acid and glycerol), and especially on carrot. The colonies are of 

 a beautiful deep rose color. On certain sugar media the color becomes 

 a poppy colored red. The yeast gives abundant growth in liquid 

 media (Raulin's solution, maltose, lactose, galactose or glycerine, 

 Raulin's solution) and especially on glycerol bouillon. The optimum 

 temperature for budding is situated between 24 and 26 C. The 

 development begins at 15 C. and stops at 38 C. to 40 C. This yeast 

 produces between 15 and 36 C. a rose-colored scum made up of elon- 

 gated cells of larger dimensions than the cells in the sediment. It 

 secretes invertase but does not ferment dextrose, maltose, lactose 

 nor d-galactose. 



PSEUDOSACCHAROMYCES STEVENSI. Anderson 2 



Anderson isolated this yeast from human feces and characterized 

 it as follows: 



" Morphology. In both young and old cultures the cells are narrowly 

 elliptical, oblong or apiculate; cytoplasm, very granular; vacuoles, 

 not distinct except in old, swollen cells; no elongated cells or false 

 mycelium are formed under any condition of culture. Budding occurs 

 only at ends, by elongation and swelling of the apiculate portion. 

 The size is 2 X 5ju. No endospores are formed. 



" Cultural Characters. On glucose agar the streak is filiform, glisten- 



1 Sartory. fitude d'une levure nouvelle, le Cryptococcus Bainieri. Comp. 

 Rend. Soc. de Biol. 61, 1906. 



2 Anderson, H. W. Yeast-like fungi of the human intestinal tract. Jour, 

 Inf. Diseases, 21 (1917), 341-386. 



