CRYPTOCOCCUS VERRUCOSUS 319 



ing, white, flat, and smooth. The growth is slow, and the colony 

 becomes dirty-gray with age. In gelatin no liquefaction occurs; 

 the growth is filiform. In beer wort and sugar mediums there is slow 

 development, with no evidence of growth except a slight sediment. The 

 giant colonies are very small. 



"Physiologic Properties. There is no fermentation of glucose, 

 levulose, sucrose, lactose, raffinose, galactose or maltose. No decided 

 change in acidity occurs in these sugars, dextrin or yeast water. There 

 is no change in litmus milk. 



CRYPTOCOCCUS VERRUCOSUS. Anderson 1 



"Morphology. In young liquid culture the cells are oblong, 

 narrowly elliptical or oblong-elongated; in old cultures elongated cells 

 are common, with several 'oil ' globules in each cell. The size is 

 3x9 microns. Budding occurs from shoulders, ends or sides. No 

 endospores are formed. 



"Cultural Characters. On glucose agar slant there is at first an 

 even, filiform, glistening, white, smooth growth; later it becomes dull, 

 brittle, verrucose and pulvinate. 

 On carrot slant the growth is more 

 profuse, with verrucose, and pul- (j "U A 

 vinate. On carrot slant the growth ^^^J^ 

 is more profuse, with verrucose ^^ 

 character more pronouned, and (j 



with chalky-white surface. There ! 



is a filiform or nodose growth in 



. . ,..'... Fig. 145-A. Cryptococcus verrucosus, 



gelatin stab, with no liquefaction. Anderson. 



On SUgar mediums and beer WOrt. 1, Cells from Young Beer Wort Culture; 2, Old 



(~^f*lls 



after 2 days, a few small, white 



patches appear on the surface, later becoming larger, dry and very 



firm; at first they remain separate, but later coalesce. 



"Physiologic Properties. It does not ferment glucose, levulose, 

 sucrose, maltose, galactose, lactose or raffinose. No decided change 

 in acidity occurs in these sugars. Litmus milk becomes very slightly 

 alkaline after several weeks. 



"The culture was isolated from human feces. 



"The dry brittle character of the colonies on solid mediums, the 

 formation of the isolated, white patches on all liquid mediums, and 

 the peculiar type of cells, clearly distinguishes this yeast from any 

 other studied." 



1 Anderson, H. W. Yeast-like fungi of the human intestinal tract. Jour. 

 Infectious Diseases, 21 (1917), 341-386. 



