352 PATHOGENIC YEASTS 



are white, becoming grayish yellow after a long time. Acid and 

 glucose gelatin is liquefied. On glycerin agar the culture is creamy 

 with indented borders. On potatoes the growth consists of a thick, 

 creamy, white layer which becomes brownish in old cultures. This 

 yeast is pathogenic for guinea pigs but not for other animals. 



CRYPTOCOCCUS HOMINIS COSTANTINI (Costantin). 



Vuillemin 



This yeast was isolated by Costantin l from a cancerous tumor 

 of the breast. It possesses round cells and is distinguished from 

 Crypt, lithogenes (San Felice) in that its cultures become brown when 

 old and from Sacch. tumefaciens (Busse) because its membranes never 

 become thick on ordinary media. 



CRYPTOCOCCUS KLEINII. Erich Cohn 



This species was discovered by Klein in milk, in which it was ac- 

 companied by various pathogenic bacteria. It has since been found 

 by Erich Cohn. 2 The cells are globular, from 2 to 6 /i in size, with 

 homogeneous contents, thin membrane, and surrounded by a hyalin 

 capsule. The capsule persists but becomes smaller in cultures. This 

 yeast is easily cultivated on beer wort agar. It does not ferment 

 dextrose, maltose, or lactose nor liquefy gelatin. 



CRYPTOCOCCUS ANOBII. Escherich 



This species was found by Escherich 3 in the cells of the intestinal 

 wall of the larva of Anobium paniceum. The cells 

 f| are pear shaped or club shaped, from 3.5 to 4 jit in 

 y size, with center provided with refractive granules 



Fi ^53 C r ypt . (Fig. 153). In culture it forms a pseudo-mycelium 



coccus Anobii (after made up of cells shaped like a sausage. 



This yeast is easily cultivated in .01% of sac- 

 charose, liquid or solid (gelatin or agar). On gelatin it gives round 

 colonies with no liquefaction. 



1 Costantin. Les levures des animaux. Bull, de Mycologie de France, v. 

 XVII, 1901. 



2 Erich Cohn. Unters. uber eine neue thierpathogene Hefeart (Hefe Klein). 

 Centr. f. Bak., v. XXXI, 1902. 



3 Escherich. Ueber das regelmassige Vorkommen von Sprosspilzen in den 

 Epithemis Kasers. Biol. Centr., v. XX, 1900. 



