ENDOMYCES FIBULIGER 371 



globules and a single nucleus. The outer part of the membrane is 

 easily detached when the conidia separate from the cells which form 

 them. The conidia have a tendency to reunite in small masses sur- 

 rounded by bubbles of air. A sort of network, mucilaginous in 

 character, is formed which is quite comparable to that formed by the 

 Saccharomycetes. This network is destroyed by heat. Possibly it 

 constitutes a means of preservation for the conidia. 



The conidia never bud in the media in which they are formed. 

 Only when transferred into a fresh medium is it that they bud either 

 by yeast-like structures or by sending out a germinating tube which 

 branches to form a mycelium. 

 They represent, then, forms 

 which are comparable to the 

 Chlamydospores of other Endo- 

 mycetes. 



In parts of the mycelium 

 which are situated in scantily 

 aerated locations, as in the sedi- 

 ment in a liquid culture or deep 

 down into a solid medium, the 



mycelium never produces conidia 



, ,_ f Fig. 160. Germination of Ascospores in 



but, on the contrary, forms a Endomyces fibuliger. 



large number of yeast-like struc- 

 tures. (Fig. 55.) These vary in their shapes and sizes. In -certain 

 media, they are smaller than conidia and resemble the Mycoderma. 

 Sometimes, they may be much larger than the conidia and possess a 

 round shape. They contain but a single nucleus. These yeasts, after 

 being detached from the mycelium for a time, continue to bud in the 

 medium in which they are formed and furnish new generations of the 

 yeasts. On fresh media, they elongate and furnish a mycelium or 

 germinate into yeast-like structures. 



Often there may be seen in parts of the mycelium that form these 

 yeast-like bodies, a sort of dissociation of filaments. The walls come 

 nearer and the units which are thus formed separate into elongated 

 cells which look like oidia. 



Lindner has noticed, in certain cases, the formation in the inter- 

 calary units of the filaments, of internal cells which he ignores, but 

 which seem to us to be conidia or yeast structures. 



The ascs are formed under the same conditions as with the Sac- 

 char omyces. They appear quickly if a piece of the mycelium, young 

 and well nourished, is placed in a covered dish containing a thin layer of 

 distilled water or on different solid media (slices of carrot) as well as in 

 most old cultures. The most favorable temperature for sporulation 



