ENDOMYCES JAVANENSIS 



373 



about a liquefaction of the gelatin after a week. The vegetation 

 is made up of a thick mycelium which gives numerous conidia. In 

 wort agar, the colonies are of a chocolate color. On carrot, this fungus 

 develops abundantly with a mycelium which in the beginning furnishes 

 many conidia; later after 18 days, there is an abundant production 

 of ascs. The deep portions of the mycelium form many yeasts. 



Endomyces fibuliger ferments saccharose actively and less actively 

 dextrose, d-mannose and levulose; it ferments feebly raffinose, lac- 

 tose, d-galactose and a-methylglucosides. It has no action on maltose, 

 dextrine, arabinose, xylose, trehalose, melibiose, mannite and inuline. 

 Endomyces fibuliger is, in general, closely related to Endomyces capsu- 

 laris. It resembles it by the complexity of its mycelium j its yeast 

 structures and the mode of formation of ascs and is distinguished only 

 by the formation of conidia and the traces of an ancestral copulation 

 which it has kept. 



ENDOMYCES JAVANENSIS. Klocker 



This species was discovered in 1909 by Klocker 1 in soil from Java. 

 The vegetation is composed in part of cells 

 like yeasts and in part of a mycelium with 

 walls. The mycelium offers a slight tendency 

 to separate its units like oidia. However, it 

 is much more reduced than in Endomyces 

 capsularis and Endomyces fibuliger (Fig. 

 161). The yeast cells (7 to 9 /* in length) 

 often look like lemons but some look like a 

 spindle; others are ellipsoidal, spherical, in 

 the form of a sausage or very much elon- 

 gated. In a general manner, they resemble 

 the cells of Saccharomyces apiculatus very 



much. The temperature limits for growth Fig ^ IQ1 . _ Endomyces Java _ 

 are: maximum 36-38 C., minimum, 5 to nen^is._ Mycelium with 

 10 C. 



Sporulation is abundant in liquid and solid 

 media as well as on plaster blocks. The 

 temperature limits for sporulation on plaster blocks are: maximum, 

 34-36 C., minimum, 5 to 10 C. 



The ascs seem to form indifferently in the yeast cells or at the 

 expense of some cell in the mycelium. They usually enclose a single 

 ascospore, rarely two. The ascospores are ellipsoidal and have the 



1 Klocker, A. L'Endomyces Javanensis, n. sp. Comp. Rend. Trav. du Lab. 

 de Carlsberg. 8, Book 4, 1909. 



nensis. 



Oidia, Yeasts and Ascs. 



A. Ascospores in Process 



of Germination (after 



Klocker). 



