SACCHAROMYCES INTERMEDIUS 



239 



Temperatures for the Formation of Ascospores 

 At 29 C. no formation of ascospores. 



27-28 appearance of first rudiments in 34 hours. 



25 " " " " " 25 



000 tl tt tt tt it O7 

 -1ITO tt tl It tt it 



1 CO It It tt tt 



11.5 " " " 



fJO It tt tt tt tl rj 



tl It It tt It -tfj 



tt 

 ti 

 it 

 u AO (( 



U n U 



0.5 no formation of ascospores. 



The ascs are often elongated and include a variable number of 

 ascospores (1 to 7) which measure 2 to 5 microns in diameter. (Fig. 

 108.) The ascospores undergo a copulation at the moment of ger- 

 mination in about half of the cases. 



Temperatures for the Formation of Scum 

 At 34 C. no formation of ascospores 



26-28 at the end of 7-10 days feeble development. 



8-15 

 " 10-25 " 



" 1-2 months 



tt 



20-22 

 13-15 " 



6-7 " 



3-5 " 



2-3 no formation of scum. 



Microscopic appearance of cells in the scum is as follows: At 

 20-28 C. the cells of the scum present 

 almost the same shape as those in 

 the sediment. One may see queer 

 shapes in the forms of chains. At 15 

 to 3 C. oval or round cells predomi- 

 nate. In the scums from old cultures, 

 the cells are almost all smaller than 

 those in the sediment. In yeast 

 water gelatin, on streaks at 15 C., 

 this yeast gives, at the end of about Fig. 108. Ascs of S. intermedius 

 16 days, a vegetation with relatively 



even edge which differs from Saccharomyces validus. It ferments dex- 

 trose, d-mannose, levulose, d-galactose, saccharose and maltose. 



