SACCHAROMYCES BLANCHARDII 271 



without capsules sometimes form in chains of two or three. The cap- 



sule may develop in old cultures. This yeast often forms ascs with 



from 1 to 4 ascospores (Busse). In gelatin stabs, 



a white growth is secured after 48 hours. The 



gelatin is not liquefied. On agar the colonies 



are small, and lunctiform and fuse quickly into 



a solid mass. On glycerol potato the growth 



is rapid. The culture has the appearance of a 



continuous dry streak, at first white and later 



brown. On liquid media, beer wort, the develop- 



ment is rapid and abundant. No scum is Fig. 124. Saccharorhy- 



formed. This yeast inverts saccharose, and 



causes a feeble fermentation. Alcohol and acid with Gelatinous Cap- 

 are formed. There is no action on maltose nor 



lactose. Its maximum temperature is around 37 C. This yeast 

 has a local pathenogenicity for the rat, the white mouse, and the dog. 



SACCHAROMYCES GRANULATUS. Vuillemin and Legrain 



This yeast was isolated by Vuillemin and Legrain. 1 It possesses 

 oval or elliptical cells, sometimes globular or elongated, about 2- 

 10 jit X 2-4 fj,. The membrane is covered with granulations which are 

 either irregular or regular. (Fig. 125.) These 

 cells form one, rarely two or three buds, and con- 

 tain fat globules of a reddish color. The cultures 

 thus take on a vermillion tint. Some of the cells 

 are able to encyst and change into durable cells 

 or chlamydospores. On plaster blocks, the cells 

 have very thin and folded membranes containing 

 g 125. _ Sarcharo- two or f ur ascospores which are spherical or 

 myces granulatus. elliptical. On liquid media this yeast does not 

 P r duce a scum b ut a sediment is formed which 

 is reddish in color. In gelatin stabs, punctiform 

 colonies are formed. The gelatin is not liquefied. On agar, beet, 

 carrot, or cabbage, it forms a folded and shiny coating. On potato 

 slants, the growth is dry. This yeast is pathenogenic for the rabbit. 



SACCHAROMYCES BLANCHARDII. (Blanchard). Guiart 2 



This yeast described by Blanchard, 3 Schwartz and Binot was iso- 

 lated from a tumor of a man. It was taken from the peritoneum after 



1 Vuillemin, P., and Legrain. Sur un cas de Saccharomycose humaine. Arch. 

 de Parasit., 3, 1900. 



2 Guiart, J. Precis de parasite-logic. Bailliere, 1910. 



3 Blanchard, R., Schwartz, E., and Binot J. Sur une Blastomycose intra- 

 peritoneale. Arch, de Parasit., 7, 1903. 



