354 PATHOGENIC YEASTS 



thick, with a reddish color. They resemble very much the cultures 

 of Bacillus prodigiosus. The cells are small and oval, from 8 to 12 fj, 

 long. This yeast is closely related to Crypt, glutinis and may per- 

 haps be a variety of it. 



CRYPTOCOCCUS NEOFORMANS. San Felice 1 



This yeast was found by San Felice on fermenting fruits. The 

 cells are of variable dimensions; some of them have a refractive 

 granule in the center. In the small cells the center is homogene- 

 ous. In the large ones one finds a central hyaline part with a very 

 refractive peripheral ring. 



This yeast develops on ordinary substances. On gelatin plates 

 the surface colonies are different from those down in the media. 

 The surface colonies are large like the head of a pin. They are 

 quite round and form projections on the surface of the media. The 

 deep colonies are somewhat spherical with a very fine contour. Gelatin 

 is not liquefied. The colonies on agar have the same appearance. 

 Growth on gelatin stabs develops as much along the line of inocula- 

 tion as along the surface. There is no liquefaction. 



This yeast is pathogenic and causes tumors in animals. 



CRYPTOCOCCUS OF CLERC AND SARTORY 2 



This species found in chronic angina has elongated oval cells, from 

 7 to 10 /-t by 5 /z in size, isolated or in groups of five or six. Budding 

 may take place at one of the ends. The cells easily take various 

 colors and are not decolorized by the Gram method of staining. The 

 optimum temperature for budding is 30 C. The yeast vegetates 

 easily on all the usual media and especially on slices of carrot. It 

 does not liquefy gelatin, coagulates milk, and ferments lactose but 

 not galactose. It secretes invertase, produces alcoholic fermentation of 

 dextrose, but does not hydrolize starch. Possessed of a very feeble 

 virulence, it is able under certain conditions, to live in the animal 

 organism and cause localized and curable lesions. 



BLASTOMYCES HESSLERI. Rettger 



Found by Rettger 3 in an abscess on the chin, this yeast develops 

 quickly in most of the media at blood heat, but contrary to ordi- 



1 San Felice, F., Ueber die pathogene Wirkung der Sprosspilze, Zugleich ein 

 Beitrag zur Aetiologie der bosartigen Geschwiilste. Centr. f. Bakt. v. XVII, 1895. 



2 Clerc, A., and Sartory, A., Etude biologique (Tune levure isolee au cours 

 (Tune angine chronique, C. R. de la Soc. de Biologic, v. XIX, 1908. 



3 Rettger, A. Contribution to the study of pathogenic yeasts, Centr. f. 

 Bak., v. XXXVI, 1904. 



