SACCHAROMYCES MEMBRANOGENES 357 



CRYPTOCOCCUS SALMONEUS. Sartory 



This species was found by Sartory 1 with Oidium lactis, in various 

 specimens of gastric contents in hyperacidity. Of 17 of these juices 

 13 gave cultures of this yeast. It is a yeast quite closely related to 

 S. rosaceus with a beautiful deep rose color in which the tint varies 

 with the temperature and the culture medium. The cells are spherical, 

 averaging from six to eight microns in diameter. 



The optimum temperature for budding is situated between 22 

 and 25 C. ; however, the yeast develops from 15 to 34. In this lat- 

 ter case the rose dolor turns to a pale tint and becomes very feeble 

 at 39. Between 40 and 41 the yeast ceases to vegetate. 



This yeast forms a rose-colored scum on glycerol broth at tempera- 

 tures between 15 and 38. The most favorable temperature for the 

 formation of scum is between 26 and 28. The cells of young scums 

 differ a little from the cells in the sediment, but in old ones they be- 

 come elongated or sausage shaped and one begins to notice structures 

 like a mycelium. The deposit at the bottom of the culture flask is 

 made up almost entirely of spherical cells only. 



Cr. salmoneus is easily cultivated on all solid media (gelatin, agar, 

 potato, carrot). It also develops easily on the various liquid media. 

 The pigment is soluble in carbon bisulfide, benzine, chloroform, ethyl 

 alcohol, ether, acetone and is insoluble in methyl alcohol. 



This yeast secretes invertose but does not produce alcoholic fer- 

 mentation. It is without action on dextrose, maltose, d-galactose, 

 starch or inulin. It precipitates caseine in 18 days but does not pep- 

 tonize the curd. It is not pathogenic for guinea pigs, rabbits and 

 dogs. 



LE DANTEC'S 2 YEAST 



It was discovered in a stool from Sprue (chronic diarrhoea of a 

 warm climate) and according to Le Dantec seems to be the cause of this 

 sickness. The yeast ferments glucose broth and does not liquefy 

 gelatin. In aerobic media it grows especially like yeasts; cultivated 

 in anaerobic media it presents somewhat the form of a mycelium. 



SACCHAROMYCES MEMBRANOGENES. Steinhaus 3 



This yeast was found by Steinhaus in a child attacked by scarlatina 

 who presented the phenomena of tracheal stenosis. Fragments of 



1 Sartory, A., Cryptococcus salmoneus, Bull, de la Soc. myc. de France, v. 

 XXIII, 1907. 



2 Le Dantec, A., Presence d'une levure dans la Sprue, C. R. Soc. Biol., v. 

 LXIV, 1908. 



3 Steinhaus, F., Untersuch. iiber eine neue Menschen und Thierpathogene 

 Hefeart, Centr. f. Bakt., v. XLIII, 1906. 



