358 PATHOGENIC YEASTS 



membrane taken from the trachea gave on inoculation the culture of 

 this yeast. 



It is a yeast with round cells, globular in shape, with a reddish 

 pigment, and sometimes it is very large. Often the cells are pear 

 shaped and bud. The bud appears at one end of the cell, which be- 

 comes pointed, but the budding is also accomplished as in other 

 yeasts at some other point on the cell. The cells have a double 

 wall, very fine and granular contents, with sometimes one or two 

 bright granules. In membranes taken from the trachea, the cells are 

 surrounded by a large capsule; this capsule appears in cultures freshly 

 taken from the organs attacked and in very old cultures, but in gen- 

 eral does not exist in cultures. 



This yeast develops easily on acid substrate, especially on agar 

 and beer wort. At the end of 12 days it forms white colonies, moist, 

 round, confluent and becoming brown in old cultures. In peptone 

 broth it causes a cloudiness and later a delicate precipitate in the 

 bottom. Still later it causes a flocculent deposit at the bottom of the 

 culture flask. On gelatin streaks it produces a white, moist growth; 

 on stabs the growth is established along the line of inoculation with 

 fine, round, isolated colonies. On the surface it forms a sort of a 

 button. On gelatin plates the colonies are round and white. On 

 dextrose agar there is a production of gas, but no gas is formed in 

 manose, maltose or saccharose agar. On beer wort the growth con- 

 sists of a dry grayish-white layer of round confluent colonies. 



This yeast is very pathogenic for rabbits. 



ATELOSACCHAROMYCES OF HUDELO. De Beurmann 

 and Gougerot 



This yeast was found by Hudelo, Duval and Loederich l in a human 

 Saccharomycosis, manifested especially by a periostitis of the tibia. 

 The cells are refractive, spherical (2-20 JJL in diameter), sometimes oval 

 or elongated into short sausage-shaped cells. No filaments are formed. 



The species grows easily in ordinary media, especially on carbo- 

 hydrate media with slight acid. The optimum temperature is situated 

 at about 22, but growth is accomplished even up to 38. On carbo- 

 hydrate agar white streaks are formed which are opaque and moist ; on 

 gelatin there is meager development with no liquefaction; on potato 

 whitish streaks are formed, later becoming ochre colored, and finally 

 a reddish black pellicle is formed. 



This yeast inverts saccharose, but does not decompose lactose. 



1 Hudelo, Duval and Loederich, Un cas de Blastomycose a foyers multiples. 

 Bull, et mem. de la Soc. de med. des hop. de Paris, 1906. 



