374 FUNGI RELATED TO THE YEASTS 



shape of a slightly flattened ball. They have but a single membrane. 

 This membrane is supplied with more or less distinct elevations. A 

 projecting ring runs around the middle and makes the ascospores look 

 like those of Willia Saturnus. However, this ring may be so placed 

 that the ascospores look like hats. Germination of the ascospores is 

 accomplished by budding in the yeasts and by the formation of ger- 

 mination tubes (Fig. 161, A). In old cultures in must, there is formed 

 on the surface of the medium and along the walls a thick ring which 

 may grow and entirely cover the surface. The least jarring of the 

 flask causes this to fall to the bottom. The giant colonies on gelatin 

 have a viscous appearance. The surface is much folded and the center 

 slightly sunken by a slight liquefaction of the gelatin. This species 

 does not invert saccharose or ferment dextrose; it acts feebly on 

 levulose. 



Endomyces javanensis constitutes a more immediate link between 

 the Saccharomycetes and the Endomycetes than some of the other Endo- 

 myces. The fact that the ascs form often from yeast cells unites it 

 very much more to the Saccharomycetes and accordingly it is advisable 

 to class it with this family. 



ENDOMYCES CRUZI. Mello and Paes * 



Hello and Paes found a yeast with oval cells (4-8 by 2-4 M) in 

 the lungs of a man of 45 years who had been asthmatic for 10 

 years. This yeast grew well on glycerol and plain potato, Raulin's 

 solution, Sabouraud's gelatin, plain and glycerol bouillon and alkalin 

 agar. On solid media the growth was a yellowish creamy white. 

 The growth was more abundant on alkalin media. This yeast fer- 

 mented glucose, maltose, dextrose and saccharose. In cultures these 

 authors observed both budding forms and mycelial cells. The ascs 

 contained from two to four spores. This fungus seemed to be closely 

 related to Endomyces vuillemini, Landrieu, 1912. The latter fungus, 

 however, prefers an acid medium and does not ferment dextrose. 



1 Mello, F. de and Paes, A. Endomyces cruzi n. sp. agent (?) d'une endomy- 

 cose bronchique simulant 1'asthme. Arquivos de Higiene e Patologia ex6ticas, 6 

 (1918) 51-60. Bull. Past. Inst. 17 (1919) 636. 



