CII. IX} LA HE I. INI,' AND STOR1\C PREPARATIONS 263 



derived. Condition of rest or activity ; fasting or full fed at the time of death. 



5. The chemical treatment, the method of fixing, hardening, dissociat- 

 ing, etc., and the time required. 



6. The mechanical treatment, imbedded, sectioned, dissected with 

 needles, etc. Date at which done. 



7. The staining agent or agents and the time required for staining. 



8. Dehydrating and clearing agent, mounting medium, cement used for 

 sealing. 



9. The objectives and other accessories (micro-spectroscope, polarizer, 

 etc.,) for studying the preparation. 



10. Remarks, including references to original papers, or to good figures 

 and descriptions in books. 



2 368. A Catalog Card Written According to this Formula : 

 Muscular Fibers. Cat. 



C. 0.15 mm. 

 Fibers 20 to 40 n thick. 



2. No. 475. (Drr. IX) Oct. i, 1891. S. H. G., Preparator. 



3. Tendinous and intra-muscular terminations of striated muscular fibers 

 from the Sartorius of the cat ( Felis domestica) . 



4. Cat eight months old, healthy and well nourished. Fasting and quiet 

 for 12 hours. 



5. Muscle pinned on cork with vaselined pins and placed in 20 per cent 

 nitric acid immediately after death by chloroform. Left 36 hours in the acid; 

 temperature 20 C. In alum water ()4 sat. aq. sol.) i day. 



6. Fibers separated on the slide with needles, Oct. 3. 



7. Stained 5 minutes with Delafield's hematoxylin. 



8. Dehydrated with 95% alcohol 5 minutes, cleared 5 minutes with carbol- 

 turpentine, mounted in xylene balsam ; sealed with shellac. 



9. Use a 16 mm. for the general appearance of the fibers, then a 2 or 3 

 mm. objective for the details of structure. Try the micro-polariscope 

 ('i 240, 248). 



10. The nuclei or muscle corpuscles are very large and numerous ; many 

 of the intra-muscular ends are branched. See S. P. Gage, Proc. Amer. Micr. 

 Soc., 1890, p. 132; Ref. Hand-book Med., Sci., Vol. V., p. 59. 



% 369. General Remarks on Catalogs and Labels. It is especially desir- 

 able that labels and catalogs shall be written with some imperishable ink. 

 Some form of water-proof carbon ink is the most available and satisfactory. 

 The water-proof India ink, or the engrossing carbon ink of Higgins, answers 

 well. As purchased, the last is too thick for ordinary writing and should be 

 diluted with one-third its volume of water and a few drops of strong ammonia 

 added. 



If one has a writing diamond it is a good plan to write a label with it on 

 one end of the slide. It is best to have the paper label also, as it can be more 

 easily read. 



