ROBERT RUSSELL BENSLEY 35 



intestinales of Lieberktihn, they empty almost exclusively into the latter. A few 

 independent ducts, however, are present. 



The large glandular tubules are composed of large cylindrical cells, 18 ju-21 fi in 

 height, surrounding a very narrow lumen. The nucleus is flattened or crescentic and 

 placed in the base of the cell. The body of the cell exhibits a faintly staining network 

 containing the secretion. Kuczynski (1890) was unable to obtain any specific staining 

 of this secretion by the methods he employed. He was also unsuccessful in attempting 

 to stain the secretion of the similar cells of the pyloric glands, although he remarks 

 that the pyloric glands near the fundus zone stain with Victoria blue. He concluded 

 that if they contain any mucin it was not their exclusive constituent. By the tech- 

 nique recommended at the beginning of this article, I have succeeded in staining the 

 secretion of both the pyloric glands and the glands of Brunner intensely in muchse- 

 matein and in mucicarmine. When so stained the meshes of the cytoplasmic network of 

 the body of the cell are found to be filled with a compact mass of small granules. 

 Staining in indulin-eosin-aurantia mixture also gave successful results. There is, 

 therefore, no adequate reason for supposing that the cells of the glands of Brunner of 

 the pig are essentially different in function from those of other mammals. 



In the sheep the differences between the glands of Brunner and the pyloric glands 

 are very striking, and I have found that even with the solutions which I have 

 employed with success on other mammals the secretion of the Brunner' s glands stains 

 with difficulty. 



The pyloric glands of the sheep are simple tubes composed of somewhat narrow 

 triangular cells 12.5 /J, to 17 /* in height with nuclei round, oval, or crescentic accord- 

 ing to the shape and secretory condition of the cell. Around the nucleus is a very 

 small amount of finely reticular cytoplasm. The body of the cell is transparent, and 

 finely reticular. The secretion forms a continuous mass, and stains readily and deep- 

 ly in muchsematein and mucicarmine. 



The glands of Brunner are exceedingly large tubules, with wide lumina sur- 

 rounded by cylindrical cells 16 /t to 22 p in height. The nucleus in these cells is 

 spherical or oval, slightly cupped on the side directed toward the lumen, and located in 

 the proximal end of the cell. The whole of the cell between the nucleus and the 

 lumen is filled with secretion which is distinctly divided into a proximal and distal 

 mass in many of the cells. The secretion stains in muchsematein, but much more 

 slowly, and less intensely, than that of the pyloric glands. The differences in shape 

 and character of the tubules and cells of the glands of Brunner are particularly obvious 

 opposite the sphincter pylori where glands of Brunner occur in the mucous membrane 

 side by side with the pyloric glands. Very striking at this point is the contrast, in 

 muchaematein preparations, between the deeply stained narrow tubules of the pyloric 

 glands and the faintly stained wide tubules of the glands of Brunner. The difference 

 in staining capacity does not, as will be pointed out later, imply a difference in the 

 amount of mucin secreted by the cells. 



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