14 L. A. JULIANELLE 



bach ** show a relation between virulence and pigmented cocci, while Passet 2b 

 and Fisher and Levy 27 show that the lightly colored or colorless forms are 

 most often associated with disease processes. 



EXPERIMENTS 



In determining chromogenesis the technic employed was that sug- 

 gested by Winslow and Winslow. 28 Cultures were grown on agar 

 slants at 20 C. for 2 weeks. A portion of the growth was spread over 

 white roughened paper, with a platinum loop and allowed to dry in air. 

 The hue and tint were matched against the colors of the frontispiece 

 of their book (table 5). 



TABLE 5 

 SOURCES AND CHROMOGENESIS OF THE STRAINS STUDIED 



A 1 From the air Lemon yellow I 



A 2 From the air Medium cadmium yellow IV 



A 3 From the air Cadmium orange III 



A 5 From the air Medium cadmium yellow IV 



F 1 From feces White 



H2 From heart's blood at necropsy Medium cadmium yellow V 



PI From pus from spine Cadmium orange IV 



P 2 From pus from carbuncle Lemon yellow I 



P 3 From pus from acne Cadmium orange IV 



P4 From pus from extracted tonsil Cadmium orange IV 



P 5 From pus (unclassified) Cadmium orange IV 



S 2 From skin White 



T 1 From throat Orange yellow III 



T 2 From throat Medium cadmium yellow V 



T 3 From throat Medium cadmium yellow VI 



T 5 From throat Lemon yellow II 



T 6 From throat Lemon yellow III 



T 8 From throat White 



T 9 From throat Orange yellow V 



X From blood culture (case ferunculous) Orange yellow III 



C15 From throat White 



C16 From throat White 



CIS From throat White 



J 1 From pus White 



L 1 From pus Cadmium orange IV 



It will be seen at a glance that there is no relationship between pig- 

 ment and hemolysis. The cultures are all hemolytic, and yet they vary 

 from a white to a rich golden brown. This is scarcely surprising. The 

 literature shows that pigment production may be varied, and while 

 the hemolytic activity seems to be fixed, it could hardly be expected that 

 the two functions would be related. 



ACID PRODUCTION IN THE PEPTONE MEDIUM OF CLARK AND LUBS 



Preparatory to the carbohydrate metabolism studies of staphylococci, 



this experiment was made to determine in a general way any relation- 







25 Dent. med. Wchnschr., 1884, 6, p. 31. 



28 Passet: Fortschr. d. Med., 1885, 33, p. 33. 



Dent. Ztschr. f. Chir., 1893, 36, p. 94. 



28 Systematic Relationships of the Coccocese, 1908. 



