6 L. A. JULIANELLE 



hemolytic alike is the same, P H 8.4. If the hemolysis were the effect 

 of reaction, all the cultures should show a like behavior on blood. But 

 since the cultures do not show the same hemolytic activity, it is reason- 

 able to exclude reaction as the cause of hemolysis. Incidentally, sterile 

 salt solution, the reaction of which is adjusted to P H 8.4, does not cause 

 hemolysis. 



Charts 1 and 2. Showing counts, amounts of amino acids and hemolytic 

 substances produced. 



12 13 14 IB 



Fig. 2. Strain AS. 



2. No effort was made to determine the tonicity of the cultures. 

 The impression was gathered from the work of Larson et al. 9 that 

 bacteria of themselves do not change the surface tension of mediums, 

 and in their study specific depressants were added when a drop in 

 surface tension was desired. 



Larson, Cantwell, and Hartzell: Jour. Infect. Dis., 1919, 25, p. 41. 



