HEMOLYTIC STAPHYLOCOCCI 



presence of hemolysins, the hydrogen-ion concentration was read on the 

 Clark and Lubs 6 scale ; the amino acidity was titrated by the Sorensen 7 

 method ; the proteose content was determined by the Vernon tests ; 8 and 

 numerical counts made at the end of each day, as long as was deemed 

 necessary for the points at hand. 



TABLE i 



PRODUCTION OF HEMOI.YSINS 



Control. 



Hemolyis Amino Acidity 

 38 



Proteose 

 0.25 



H-ion 

 7.5 



Figures for proteose content represents amount of medium required to equal 1 c c ol 

 standard. 



Figures for amino acidity represent c c of 20/N NaOH required to neutralize 100 c c of 

 medium. 



Plus signs indicate: +, 25% hemolysis; ++, 50%; + ++, 75%. 



Bacterial Counts Made With Production ol Hemolysin Stains 



1. An analysis of the results (table 1 and chart 1) shows several 

 points : The ultimate reaction of all the cultures hemolytic and non- 



Jour. Bacteriol., 1917, 2, p. 109. 



7 Biochem. Ztschr., 1908, 7, p. 45. 



8 Jour. Physiol., 1904, 30, p. 330. 



