64 



MINNESOTA BOTANICAL STUDIES. 



Chloroform. Vapors of chloroform seem to be very in- 

 jurious to resting bulbs. The following quantities of the re- 

 agent were used : i/iooo, 1/5000 and i/ioooo part in air and 

 in all three cases the bulbs were killed. The bulbs used 

 were of the same kind as in the experiments with am- 

 monia. The bulb decayed invariably from the shoot area to- 

 ward the center of the bulbs and never from the root area. 

 The outer portion of the bulb looked perfectly natural. The 

 growing point of the shoot was killed in every case. These 

 results are of but little value since the temperature of the plant 

 house fell to 5 C. during one night. 



Alcohol. This reagent seemed to arrest growth. Experi- 

 ments were set up containing i/iooo and 1/500 parts of alcohol 

 in 4000 cc. of air. The bulbs were kept under the bell jars for 

 10 days and when taken out and potted they were perfectly 

 natural. The root areas had begun to swell. No discolora- 

 tion was noticeable. 



TABLE VI. 



I. i/iooo part alcohol in 4000 of air. 

 II. 1/500 part of alcohol in 4000 of air. 

 III. Control. 



The above table shows a peculiarly interesting result. In the 

 experiment where i/iooo part of alcohol was used no ill effect 

 on the plants could be detected. The result is more striking in 

 the experiment where 1/500 part of the alcohol was used. The 

 bulbs remained almost stationary and up to February 19, or 100 

 days from the time the bulbs were placed under the bell jars, the 

 total growth was only 50 mm Upon an examination it was found 

 that the root system was perfectly natural and well developed, 

 completely filling the four-inch pots into which the bulbs were 



