202 MINNESOTA BOTANICAL STUDIES. 



portion probably proteid. In sections stained heavily with 

 aniline blue the central portion appears blue. Around the clear 

 center are arranged 5-10 plates of starch which stain brown 

 with both a KI and an alcohol solution of iodine. By careful 

 washing of material stained in an alcoholic solution of iodine 

 and with the aid of a -^ oil immersion lens a distinct violet 

 tinge is discernible in the plates. The protoplasmic contents of 

 the cell are usually most dense in the pointed part. Between 

 the chromatophore and the cell wall are found numerous 

 rust-brown to black (in a few cases copper-colored) bodies 

 of different form and size. In some places they occur in diffuse 

 patches the limits of which are often indefinable, in others as 

 five-pointed rosettes. Again they may appear filiform, partially 

 and usually irregularly coiled or forming a delicate and loose 

 network. I have interpreted these bodies as products of the 

 action of the dilutely acid formalin solution and as identical 

 with the hypochlorin of Pringsheim. His plates agree closely 

 with much of the material at hand. In accordance with 

 Pringsheim's account of the chemical reactions of hypochlorin, 

 these brown bodies are wanting in those sections which have 

 been carried through the alcohols in the method for paraffin 

 embedding. 



A large amount of material has been examined but in no 

 case has even a trace of the production of zoospores or gametes 

 been found. The stages in the penetration of the nurse plant, 

 consisting in the elongation of the at first spheroidal cell, the 

 subsequent withdrawal of the protoplasm into the inner end and 

 the increase in size of the latter to form the mature pyriform 

 cell, have been observed, but nothing to indicate the formation 

 of zoospores. 



Conclusions. It is therefore upon the basis of vegetative 

 characters that the endophyte described above is provisionally 

 placed in the genus Chlorochytrium under C. inclusum Kjell- 

 man. Upon examination of the Chlorochytrium inclusum found 

 upon Dilsea (Sarcophyllis} distributed in Phycotheca Boreali- 

 Americana (Fasc. XL, no. 514) this is seen to possess a 

 thicker cell wall than the material on Constantinea sitchensis, is 

 almost spheroidal, larger, has denser dark green contents, con- 

 tains no pyrenoids (or very inconspicuous, if present at all) and 

 resembles a resting stage. The time of collection, December, 

 moreover, strengthens this last supposition. The material under 



