XL. CORALLINE VER^ OF PORT RENFREW. 



K. YENDO, Rigakushi, 

 SCIENCE COLLEGE, IMPERIAL UNIVERSITY, TOKYO. 



I had the opportunity of studying seaweeds during the sum- 

 mer of 1901, at the Minnesota Seaside Station at Port Ren- 

 frew, Vancouver Island, B. C. The vicinity of the laboratory 

 is extremely rich in marine life and afforded a good number 

 of examples. I paid special attention to calcareous algae, in 

 which branch I have been deeply interested. Returning with 

 the specimens to the Botanical Institute of the University of 

 Tokyo, I carefully examined the Corallines (verae) and pre- 

 pared the present paper. Other specimens belonging to the 

 subfamily Melobesiae have been sent to Mr. M. Foslie, of Nor- 

 way ; his paper should also appear in a short time. 



The material was partly dried and partly preserved in alco- 

 hol, the accompanying photographs being taken from the latter. 

 The sections were made from alcoholic material by microtome, 

 after decalcifying in Pereny's fluid. Amphfroa tuberculosa and 

 a few other thick plants were not satisfactorily decalcified by 

 the solution and the author found the following mixture specially 

 suited for the purpose : 



Hydrochloric acid, 5 per cent 40 c.cm. 



Alcohol, absolute 30 c.cm. 



Chromic acid, 0.5 per cent 30 c.cm. 



The sections were stained after my usual method. One 

 brings down the sections to pure water, and stains with Boemer's 

 haematoxylin for 2040 minutes ; treat with acetic acid if neces- 

 sary, and then dip in fuchsin (0.3 gr. in 100 c.cm. of 50 per 

 cent, alcohol for one hour; 90 per cent, alcohol, abs. alcohol, 

 xylol, balsam. The spores and spore-forming cells stain in red 

 and the cell-walls of the vegetative cells in purple. 



711 



