252 THE BLOOD. 



shown that a contamination of the serum-globulins with soap can essentially 

 modify the precipitation of these globulins. Under these circumstances the above 

 assumptions in regard to the different globulin fractions must be accepted with 

 great caution. 



The investigations made thus far upon the so-called serglobulin 

 have not led to any positive results. That this globulin, with the excep- 

 tion of the enzymes, immune bodies, and other unknown substances 

 which are carried down by the various fractions, is a mixture of globulins 

 there seems to be no doubt. The serglobulin or the globulin mixture 

 which is obtained from the serum by the methods to be described has the 

 following properties : 



In a moist condition it forms snow-white flaky masses, neither tough 

 nor elastic, which always contain thrombin and hence can bring about 

 coagulation in a fibrinogen solution. The neutral solution is only incom- 

 pletely precipitated by NaCl added to saturation, and is not precipitated 

 by an equal volume of a saturated salt solution. It is only partly pre- 

 cipitated by dialysis or by the addition of acid. On saturation with 

 magnesium sulphate or one-half saturation with ammonium sulphate 

 a complete precipitation is obtained. The coagulation temperature 

 is, with 5-10 per cent NaCl in solution, 69-76, but more often 75 C. 

 The specific rotation of the solution containing salt is (a) D = 47.8 for 

 the serglobulin from ox-blood (FREDERICQ J ). The various globulin 

 fractions do not differ essentially from each other in their coagulation 

 temperatures, specific rotation, refraction coefficient (REiss 2 ), and their 

 elementary composition. The average composition is, according to 

 HAMMARSTEN, C 52.71, H 7.01, N 15.85, S 1.11 per cent. K. MoRNER 3 

 found 1.02 per cent sulphur and 0.67 per cent lead-blackening sulphur. 

 All the sulphur seems to exist as cystine. 



Serglobulin contains, as K. MORNER first showed, a carbohydrate 

 group which can be split off. LANGSTEIN 4 has obtained several car- 

 bohydrates from the blood-globulin, namely, dextrose, glucosamine, 

 and carbohydrate acids of unknown kinds. It has not been shown 

 whether these small amounts of carbohydrate are derived from the globulin 

 or from other contaminating bodies. According to ZANETTI and also 

 BYWATERS, the blood-serum contains a glucoproteid, seromucoid, and 

 the investigations of EICHHOLZ S seem to show that the globulins are 



1 Bull. Acad. Roy. de Belg. (2), 50. In regard to paraglobulin, see Hammarsten, 

 Pfliiger's Arch., 17 and 18, and Ergebnisse d. Physiol., 1, Abt. 1. 



3 Hofmeister's Beitrage, 4. 



3 Zeitschr. f. physiol. Chem., 34. 



4 M6rner, Centralbl. f. Physiol., 7; Langstein, Munch, med. Wochenschr., 1902, 

 1876, and Wien. Sitzungsber., 112, Abt. 116, 1903; Monatsheft f. Chem., 25; Hof- 

 meister's Beitrage, 6; see also footnote 3, p. 83. 



5 Zanetti, Chem. Centralbl., 1898, I, p. 624; Bywaters, Journ. of Physiol., 35, and 

 Bicohem. Zeitschr., 15; Eichholz, Journ. of Physiol., 23. 



