270 THE BLOOD. 



Hemoglobin from the C H N S Fe O P 2 O 5 



Dog 53.85 7.32 16.17 0.390 0.430 21.84 (HOPPE-SEYLER) 



" 54.577.2216.3,80.5680.33620.93 (JAQUET) 



Horse 54.87 6.79 17.31 0.650 0.470 19.73 (KOSSEL) 



" 51.156.7617.940.3900.33523.43 (ZINOFFSKY) 



Ox 54.667.2517.700.4470.40019.543 .... (HUFNER) 



Pig 54.177.3816.230.6600.43021.360 (OTTO) 



" 54.71 7.38 17.43 0.479 0.399 19.602 (HUFNER) 



Guinea-pig 54.12 7.36 16.78 0.580 0.480 20.680 (HOPPE-SEYLER) 



Squirrel 54.09 7.39 16.09 0.400 0.590 21.440 



Goose 54.26 7.10 16.21 0.540 0.430 20.690 0.770 



Hen 52.47 7.19 16.45 0.857 0.335 22.500 0.197 (JAQUET) 



That the repeatedly observed quantity of phosphorus in the haemo- 

 globin of birds (Inoko and others) is due to a contamination has been 

 proven by ABDERHALDEN and MEDIGRECEANU. In the haemoglobin 

 from the horse (ZINOFFSKY), the pig, and the ox (HUFNER) we have 

 1 atom of iron to 2 atoms of sulphur, while in the haemoglobin from the 

 dog (JAQUET) the relation is 1 to 3. From the data of the elementary 

 analysis, as also from the amount of loosely combined oxygen, HUF- 

 NER 1 has calculated the molecular weight of dog-hsemoglobin as 14,129, 

 and the formula CeaeHuEsNie^eSsOigi. According to the more recent 

 determinations of HUFNER and JAQUET, 2 ox-hsemoglobin contains an 

 average of 0.336 per cent iron, from which a molecular weight of 16,669 

 may be calculated. HUFNER and GANSSER S have attempted to learn 

 the size of the molecular weight of haemoglobin by means of osmotic 

 pressure determinations, and they found the following approximate 

 results: for horse haemoglobin 15,115 and for ox-haemoglobin 16,321. 

 The haemoglobin from various kinds of blood not only shows a diverse 

 constitution, but also a different solubility and crystalline form, and a 

 varying quantity of water of crystallization; hence we infer that there 

 are several kinds of haemoglobin. BOHR is a very zealous advocate 

 of this supposition. He has been able to obtain haemoglobins from dog- 

 and horse-blood, by fractional crystallization, which had different powers 

 of combining with oxygen and contained different quantities of iron. 

 HOPPE-SEYLER had already prepared two different forms of haemoglobin 

 crystals from horse-blood, and BOHR concludes from all these observa- 

 tions that the ordinary haemoglobin consists of a mixture of different 

 haemoglobins. In opposition to this statement, HUFNER 4 has shown 



1 Hoppe-Seyler, Med. chem. Untersuch., 370; Jaquet, Zeitschr. f. physiol. Chem., 

 14, 296; Kossel, ibid., 2, 150; Zinoffsky, ibid., 10; Hiifner, Beitr. z. Physiol., Festschr. 

 f. C. Ludwig, 1887, 74-81, Journ. f. prakt. Chem. (N. F.), 22; Otto, Zeitschr. f. physiol. 

 Chem., 7; Inoko, ibid., 18; Abderhalden and Medigreceanu, ibid., 59. 



2 Arch. f. (Anat. u.) Physiol., 1894. 



3 Arch. f. (Anat. u.) Physiol., 1907. 



4 Bohr, " Sur les combinaisons de 1'hemoglobine avec 1'oxygene," Extrait du 

 Bulletin de 1' Academic Royale Danoise des sciences, 1890; also Centralbl. f. Physiol., 

 1890, 249. Hoppe-Seyler, Zeitschr. f. physiol. Chem., 2; Hiifner, Arch. f. (Anat. u.) 

 Physiol., 1894. 



