PUS. 345 



ferment. According to the analyses of HOPPE-SEYLER x the pus-serum 

 contains in 1000 parts: 



i. n. 



Water 913.70 905.65 



Solids 86.30 94.35 



Proteins 63.23 77.21 



Lecithin 1 . 50 . 56 



Fat 0.26 0.29 



Cholesterin , 0.53 0.87 



Alcohol extractives 1 . 52 . 73 



Water extractives 11.53 6.92 



Inorganic salts 7 . 73 7 . 77 



The ash of pus-serum has the following composition, calculated to 

 1000 parts of the serum. 



i. ii. 



NaCl 5.22 5.39 



Na 2 SO 4 0.40 0.31 



Na 2 HPO 4 0.98 0.46 



Na 2 CO 3 0.49 1 . 13 



Ca 3 (PO 4 ) 2 0.49 0.31 



Mg 3 (PO 4 ) 2 . 19 . 12 



PO 4 (in excess) 0.05 



The pus-corpuscles are generally thought to consist chiefly of emi- 

 grated white blood-corpuscles, and their chemical properties have there- 

 fore been given in discussing these. The molecular granules, fat- 

 globules, and red blood-corpuscles are considered rather as casual form- 

 elements. 



The pus-cells may be separated from the serum by centrifugal force, 

 or by decantation directly or after dilution with a solution of sodium 

 sulphate in water (1 vol. saturated sodium-sulphate solution and 9 vols. 

 water), and then washed by this same solution in the same manner as 

 the blood-corpuscles. 



The chief constituents of the pus-corpuscles are proteins, of which 

 the largest portion seems to be a nucleoprotein which is insoluble in 

 water and which expands into a tough, slimy mass when treated with a 

 10-per cent common-salt solution. This protein substance, which is 

 soluble in alkali but is quickly changed thereby, is called ROVIDA'S hyaline 

 substance, and the property of the pus of being converted into a slime- 

 like mass by a solution of common salt depends on this substance. Besides 

 this substance, to which the nucleoprotein of the pus-cells investigated 

 by STRADA 2 seems to stand in close relation, we also have a globulin 

 which coagulates at 48-49 C., as well as serglobulin (?), seralbumin, 

 a substance similar to coagulated protein (MIESCHBR), and lastly peptone 



Med.-chem. Untersuch., 490. 2 Bioch. Zeitschr., 16. 



