348 CHYLE, LYMPH, TRANSUDATES AND EXUDATES. 



woman OIDTMANN found 714.32 p. m. water, 284.5 p. m. organic and 

 1.16 p. m. inorganic substances. In the cells of the mesenteral lymphatic 

 glands of oxen BANG 1 found 804.1 p. m. water, 195.9 p. m. solids, 137.9 

 total proteins, 6.9 p. m. histone nucleate, 10.6 p. m. nucleoprotein, 47.6 

 p. m. bodies soluble in alcohol, and 10.5 p. m. mineral constituents. 



The Thymus. The colls of this gland are very rich in nuclein bodies 

 and relatively poor in the ordinary proteins, but their nature has not been 

 closely studied. The chief interest is attached to the nuclein substances. 

 KOSSEL and LILIENFELD first prepared from the watery extract of the 

 gland, by precipitating with acetic acid and then further purifying, a 

 protein substance which has been generally called nudeohistone. By the 

 action of dilute hydrochloric acid upon nudeohistone it splits, according 

 to these investigators, into histone and leuconuclein. The leuconuclein 

 is a true nuclein ; hence it is a nucleic-acid compound with protein which 

 is relatively poor in protein and rich in phosphorus. The more recent 

 investigations of BANG, MALENGREAU, and HuiSKAMP 2 upon nudeo- 

 histone all show that this nucleoprotein is not a unit substance, but a 

 mixture of at least two bodies. The views of the investigators mentioned 

 differ quite essentially from one another as to the nature of these bodies, 

 but this is partly due to the different methods used by them and partly 

 to the ready changeability of the substances in question. 



Besides the real nudeohistone, B-nucleoalbumin of MALENGREAU, 

 LILIENFELD'S histone contains a second nucleoprotein which BANG and 

 HUISKAMP call simple nucleoprotein, while MALENGREAU designates 

 it A-nucleoalbumin. This protein, which contains only about 1 per cent 

 phosphorus and which is possibly identical with the nucleoprotein found 

 by LILIENFELD in the thymus, yields a nuclein, but no free nucleic acid, 

 on cleavage. As a second cleavage product it yields, according to MAL- 

 ENGREAU, the A-histone, which can be readily precipitated by magnesium 

 and ammonium sulphates from the ordinary B-histone of the thymus 

 gland. The occurrence of A-histone in the gland has been verified by 

 BANG, and according to BANG and HUISKAMP the A-histone is not derived 

 from the nucleoprotein, as these investigators claim that it yields no his- 

 tone. According to BANG the nucleoprotein yields only an albuminate, 

 besides the nuclein, as cleavage products. 



The true nudeohistone, which is much richer in phosphorus (the 

 calcium salt containing, according to BANG, on an average 5.23 per cent 

 P), yields ordinary histone as one cleavage product and free nucleic acid 



M. c. 



2 Lilienfeld, Zeitschr. f. physiol. Chem., 18; Kossel, ibid., 30 and 31; Bang, ibid., 

 30 and 31. See also Arch. f. Math, og Naturvidenskab, 25, Kristiania, 1902, and 

 Hofmeister's Beitrage, 1 and 4; Malengreau, La Cellule, 17 and 19; Huiskamp, Zeit- 

 schr. f. physiol. Chem., 32, 34, and 39. 



