350 CHYLE, LYMPH, TRANSUDATES AND EXUDATES. 



dry substance, and guanine. The bodies thymine and (uracil ?} obtained,, 

 besides lysine and ammonia, by KUTSCHER, as products of jautodigestion 

 of the gland, probably have a similar origin. LILIENFELD l found 

 inosite and protagon in the cells of the thymus. Among the enzymes, 

 besides arginase, guanase, and adenase, we must especially mention the 

 enzyme studied by JoNEs, 2 which acts like a nuclease. splitting off phos- 

 phoric acid and purine bases, from the nucleoproteins. This enzyme, 

 contrary to trypsin, acts best in acid liquids, and is readily destroyed by 

 alkalies at body temperature. The quantitative composition of the 

 lymphocytes of the thymus of a calf is, according to LILIENFELD' s analysis, 

 as follows. The results are given in 1000 parts of the dried substance : 



Proteids 17.7 



Leuconuclein 687 . 9 



Historic 86 . 7 



Lecithin 75 . 1 



Fat 40.2 



Cholesterin 44 . 



Glycogen 8.0 



The dried substance of the leucocytes amounted to an average of 

 114.9 p. m. Potassium and phosphoric acid are prominent mineral 

 constituents. LILIENFELD found KH 2 PO 4 among the bodies soluble in 

 alcohol. 



Attention must be called to the analyses of BANG, S which show that 

 the thymus contains about the same quantity of nucleoprotein, but about 

 five times as much histone nucleate as the lymphatic glands calculated 

 in both cases upon the same amount of dry substance. OIDTMANN 4 

 found 807.06 p. m. water, 192.74 p. m. organic and 0.2 p. m. inorganic 

 substances in the gland of a child two weeks old. 



The Spleen. The pulp of the spleen cannot be freed from blood. 

 The mass which is separated from the spleen capsule and the structural 

 tissue by pressure, and which ordinarily serves as material for chemical 

 investigations is, therefore a mixture of blood and spleen constituents. 

 For this reason the proteins of the spleen are little known. The nucleo- 

 protein isolated by LEVENE and MANDEL 5 is to be considered as a true 

 spleen constituent, and this nucleoprotein yields 25 per cent glut ami c 

 acid on hydrolysis. Histone has not been directly detected in the spleen; 

 but its presence is to be admitted because KRASNOSSELSKY 6 was able 

 to isolate a histone-peptone as sulphate from the spleen. The ferruginous 



1 Kutscher, Zeitschr. f. physiol. Chem., 34; Lilienfeld, ibid., 18. 



2 Zeitschr. f. physiol. Chem., 41. 

 3 1. c., Arch. f. Math., etc. 



4 Cited from v. Gorup-Besanez, Lehrb. d. physiol. Chem., 4. Aufl., p. 732. 



5 Bioch. Zeitschr.,, 5. 



8 Zeitschr. f . physiol. Chem., 49. 



