PART VI. 



APPENDIX. 

 CHAPTER XXL 



LABORATORY WORK. 



Laboratory work should, so far as possible, accompany the study 

 of the text. It is impossible to make a series of experiments that will 

 closely follow the order of topics in the text, since a large amount of pre- 

 liminary work has to be done in making media before actual study of 

 bacteria begins. The order of experiments below given will be found a con- 

 venient one'to follow, but may be modified to suit convenience. 



APPARATUS NEEDED. 



Steam sterilizer. 



Autoclave. 



Hot air sterilizer A common gas oven used for cooking will do. 



Stew-pan for cooking media. 



Flasks. Liter and half-liter. 



Test-tubes, heavy Board of Health pattern. 



Petri dishes four inches in diameter. 



Pipettes i c.c. and 2 c.c. Some larger ones are also convenient. 

 Each of the smaller ones should have a glass tube holder (Fig. 56) or there 

 should be a metal box with cover to hold fifty or more pipettes at once. 



Wire baskets to hold test-tubes. 



Test-tube racks, or common tumblers with a little cotton in the bottom 

 to hold test-tubes. 



Beakers. 



Evaporating dishes. 



Fermentation tubes. 



Burette holder with four burettes. 



Evaporating dishes. 



Measuring cylinders i liter and 100 c.c. 



Counting plate or counting card. 



Platinum wire to be fused into glass rods. 



Culture oven with constant temperature of 98. 



Bunsen burners. 



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