6 CONTENTS 



,PAGE 



teriolysins, 341 Specificity of bacteriolysins, 341. Practical Applications, 341 

 Technic of the Pfeiffer test, 342 Bacteriolytic test in vivo for the identification 

 of bacteria, 343 Bacteriolytic test in vivo in the diagnosis of disease, 348 Bac- 

 teriolytic test in vitro (method of Stern and Korte), 349 Bacteriolytic test in 

 vitro (method of Wright), 335. 



CHAPTER XX. HEMOLYSINS 361 



Historic, 361 Definition, 362 Nomenclature, 363 Nature of hemolysins, 

 363 Analogy between bacteriolysis and hemolysis, 367 Specificity of hemoly- 

 sins, 368 Normal hemolysins, 368 Production of immune hemolysins, 371 

 General properties of hemolysins, 371 Source of hemolysins, 372. Practical 

 Applications, 373 Quantitative reactions between hemolytic amboceptor and 

 complement, 374 Method of titration of hemolysin, 375 Method for removing 

 hemolysin from a serum, 378 Method of determining natural hemolysins in 

 serum, 378 The serum diagnosis of paroxysmal hemoglobinuria, 379 Method 

 of determining the resistance of red blood-corpuscles, 380. 



CHAPTER XXI. VENOM HEMOLYSIS 383 



Historic nature of venom hemolysis, 383 Venom hemolysis in syphilis, 

 385 Practical value of the venom test in syphilis, 388 The psycho-reaction of 

 Much in syphilis, 388 Venom hemolysis in tuberculosis, 390 Venom hemolysis 

 in cancer, 390. 



CHAPTER XXII. PRINCIPLES OF THE PHENOMENON OF COMPLEMENT FIXATION 391 



Historic, 391 The original complement-fixation method of Bordet, 394 

 Mechanism of complement fixation, 395 Non-specific complement fixation, 

 396 Quantitative factors in complement-fixation tests, 397 Practical applica- 

 tions, 399. 



CHAPTER XXIII. THE TECHNIC OF COMPLEMENT-FIXATION REACTIONS 401 



The Wassermann Syphilis Reaction, 401 Historic, 401 Principles and 

 theories of the syphilis reaction, 404 General technic, 404 Preparation of the 

 fluid to be tested, 408 Preparation and titration of complement, 411 Prepara- 

 tion and titration of hemolytic amboceptor, 415 Preparation of blood-cor- 

 puscles, 416 Preparation and standardization of antigens, 417 Technic of the 

 First Method, 435 Technic of the Second Method, 441 Technic of the Third 

 Method, 443 Technic of the Fourth Method, 446. Modifications of the 

 Wassermann Reaction, 449 Technic of the Noguchi modification, 449 Technic 

 of the Hecht-Weinburg modification, 457 Other modifications, 458. The Was- 

 sermann Reaction in the Various Stages of Syphilis, 459 The specificity of the 

 Wassermann reaction, 465 The effect of treatment upon the Wassermann re- 

 action, 466 The Practical Value of the Wassermann reaction, 469. 



CHAPTER XXIV. COMPLEMENT-FIXATION REACTIONS (continued) 473 



Specific complement fixation in bacterial diseases, 473 Preparation of 

 bacterial antigens, 473 Standardizing bacterial antigens, 475 Principles of 

 complement fixation with bacterial antigens, 476. Complement Fixation in 

 Gonococcus Infections, 477. Complement Fixation in Glanders, 484. Com- 

 plement Fixation in Contagious Abortion, 486. Complement Fixation in 

 Dourine, 487. Complement Fixation in Typhoid Fever, 489. Complement 

 Fixation in Tuberculosis, 490. Complement Fixation in the Standardization of 

 Immune Serums, 491. Complement Fixation in Echinococcus Disease, 492. 

 Complement Fixation in the Differentiation of Proteins (blood-stains, meats, 

 bacteria), 494. Complement Fixation in Cancer, 499. 



CHAPTER XXV. CYTOTOXINS 502 



Nomenclature, 502 Nature and general properties of cytotoxins, 502 

 Preparation of cytotoxins, 503 Methods of studying cytotoxins, 503 Specificity 

 of cytotoxins, 504 Autocytotoxins, 505 Isocytotoxins, 506 Anticytotoxic 

 serums, 506 Kinds of cytotoxins, 506 Spermatotoxin, 506 Epitheliotoxin, 

 506 Leukotoxin, 506 Nephrotoxin, 506 Hepatotoxin, 507 Gastrotoxin, 

 507 Syncytotoxin, 507 Neurotoxin, 508 Thyrotoxin, 508 R61e of cyto- 

 toxins in immunity, 508 Practical applications, 508 Cytotoxic cancer re- 

 action, 509. 



