56 TECHNIC OF ANIMAL INOCULATION 



4. Raise a small fold of skin with a pair of forceps, and make a 

 tiny incision through the skin with a pair of sharp-pointed scissors. 



5. With a probe, separate the skin from the underlying muscles to 

 form a funnel-shaped pocket. 



6. By means of a fine-pointed forceps or a glass tube syringe in- 

 troduce the inoculum into this pocket and deposit it as far as possible 

 from the point of entrance of the instrument. 



7. Close the wound with collodion and cotton. A single stitch with 

 fine thread may be necessary. 



METHOD OF MAKING INTRAMUSCULAR INOCULATION 



1. These injections are usually made into the posterior muscles of 

 the thigh or into the lateral thoracic or abdominal muscles. 



2. Clip away the hair over the selected area, cleanse, etc., as for 

 subcutaneous injection. 



3. Steady the skin over the selected muscles with the slightly sep- 

 arated left forefinger and thumb. 



4. Thrust the needle of the syringe quickly into the muscular tissue, 

 and slowly inject the fluid. 



METHOD OF MAKING INTRAVENOUS INOCULATION 

 Rabbit. 1. The posterior auricular vein along the outer margin 

 of the ear is better adapted than a median vein for this purpose. 



2. If a number of injections are to be made, commence as near the 

 tip of the ear as possible, as the vein may become occluded with thrombi, 

 and subsequent inoculations may then be given nearer and nearer the 

 root of the ear. 



3. The animal should be held firmly, as the slightest movement may 

 result in piercing the vein through and through and require reinsertion 

 of the needle. This is accomplished satisfactorily by placing the rabbit 

 upon the edge of the table and holding it firmly there by grasping the 

 neck and front quarters, the assistant at the same time compressing 

 the root of the ear with the thumb and forefinger. 



4. If the hair is long, clip it. 



5. The ear is struck gently with the fingers and washed with alcohol 

 and xylol; the friction will render the vein more conspicuous. 



6. The ear is grasped at its tip and stretched toward the operator, 

 or the vein may be steadied by rolling the ear gently over the left in- 

 dex-finger and holding it between the finger and thumb. 



