PRODUCTION OF ANTITOXINS FOR THERAPEUTIC PURPOSES 233 



plug them. The mixtures are made in the barrel of the syringe, and 

 sufficient sterile salt solution is placed in the side-arm to bring the total 

 volume of the injection up to 4 c.c., and to wash in all traces of toxin and 



FIG. 71. A KITCHENS SYRINGE. 



The needle is plugged by dipping the tip in carbolized vaselin. The side arm 

 holds sterile salt solution; when the needle has been entered, the injection is given by 

 pressure on the bulb; the side arm is then turned upward, when the contents flow 

 into the main barrel, and injected in this manner insures accuracy in dosage and 

 uniform bulk of inoculum. 



antitoxin. The mixtures are allowed to stand for at least fifteen min- 

 utes (Park) before being injected (Fig. 72). The pigs must be of proper 

 weight i. e., about 250 to 300 grams; the abdominal wall is shaved, 



FIG. 72. A BATTERY OF KITCHENS SYRINGES. 



and the injection given directly in the median abdominal line. The 

 animals are placed two in a cage, and carefully observed for four or 

 five days for symptoms of toxemia and edema about the site of injection. 



