456 THE TECHNIC OF COMPLEMENT-FIXATION REACTIONS 



be taken in making the test with one patient's serum. Of course, any 

 number of serums may be examined with the same controls (Fig. 116). 



TABLE 16. NOGUCHI MODIFICATION OF THE WASSERMANN 



REACTION 



At the end of the second incubation, or after two hours more at room 

 temperature, the tubes are inspected. The antigen and hemolytic 

 system controls, as well as all the rear tubes or serum controls, should 

 be completely hemolyzed. The first tube containing a known syphilitic 

 serum shows inhibition of hemolysis; the front tube containing normal 

 serum is completely hemolyzed; the front tube containing the patient's 

 serum shows complete inhibition of hemolysis (strong positive), varying 

 degrees of inhibition (moderately or weakly positive), or is completely 

 hemolyzed (negative). The results may be recorded and reported after 

 the same manner described on p. 440. 



MODIFICATION OF BAUER 



Bauer does not use rabbit-sheep amboceptor, but takes advantage 

 of the antisheep amboceptor normally present in variable amounts in a 

 large proportion of human serums. Although this test is quite delicate, 

 the quantity of natural amboceptor in human serums is too variable a 

 factor to be depended upon, and the modification is not, therefore, in 



general use. 



1 Four drops if serum is inactivated. 



