COMPLEMENT-FIXATION TEST IN CANCER 501 



The following controls are included : 



1. The fifth tube is the serum control. 



2. A known positive serum should be included and set up in the same 

 manner. 



3. A known normal serum should be used. 



4. The antigen control, containing at this time antigen, complement, 

 and salt solution. 



5. A hemolytic control, containing at this time complement and salt 

 solution. 



Each tube is gently shaken and incubated for an hour, after which 

 l]/2 to 2 units of amboceptor and 1 c.c. of corpuscles are added to each 

 tube. After one to two hours' incubation, the results are read, inhibition 

 of hemolysis with satisfactory controls indicates a positive reaction. 

 With these antigens, it is not strange that positive reactions may occur 

 in syphilis. 



