816 EXPERIMENTAL INFECTION AND IMMUNITY 



(f) What antibodies do you suspect are present in these immune 

 serums? 



EXPERIMENT 2. PRODUCTION OF PRECIPITINS * 



1. Immunize a rabbit with sterile horse serum by intravenous injections after the 

 first method as described on page 70; immunize a second rabbit after the second 

 method. 



2. Immunize two rabbits with sterile human serum after the first method. 



3. Immunize two rabbits with cow milk by intravenous injections after the third 

 method. 



4. Bleed the animals from the carotid artery one week after the last injection and 

 preserve the serums in a sterile condition. 



EXPERIMENT 3. PRODUCTION OF HEMOLYSINS 



1. Immunize a rabbit with washed sheep corpuscles by intravenous injections 

 after the first method as described on page 72. 



2. Immunize a second rabbit with sheep cells after the second method. 



3. Immunize a third rabbit with washed human cells after the third method. 



4. Immunize a fourth rabbit with washed human cells by intraperitoneal in- 

 jections as described on page 73. 



5. Bleed the animals in four days to a week after the last injection and separate 

 the serums. 



6. Prepare a portion of human amboceptor dried on paper as described on page 

 70. 



7. Preserve the balance of the serum and the other serums with equal parts of 

 neutral glycerin. 



EXPERIMENT 4. PRODUCTION OF CYTOTOXIN 



1. Immunize two rabbits with dog kidney after the method described on page 73. 



2. After the immunization has been completed, preserve the serum with 0.2 per 

 cent, phenol in sterile ampules. 



While these various immune serums are being prepared the student is 

 engaged with the viork on infection, or if the subject of immunity is taken 

 up at once, immune serums should be furnished by the instructor. 



EXERCISE 2. INFECTION 

 EXPERIMENT 5. EXPERIMENTAL PNEUMONIA 



1. Grow a virulent culture of pneumococcus in glucose serum broth for forty- 

 eight to seventy-two hours at 37 C. until a good rich growth is secured. Prepare 

 and stain smears by Gram's method. 



2. Pass a large catheter which has its tip cut off into the trachea of a dog until it 

 has passed into one of the primary bronchi. By means of a syringe inject quickly 

 15 c.c. of culture; remove the catheter and mouth-gag. Take the rectal temperature 

 and leukocyte count previous to inoculating. 



3. Inject a second dog in the same manner with 2 c.c. of culture. 



4. Inject a third dog with 5 c.c. of culture intravenously. 



