WASSERMANN AND NOGUCHI REACTIONS 859 



EXERCISE 41. NOGUCHI MODIFICATION OF THE WASSERMANN RE- 

 ACTION 



EXPEKIMENT 92. TlTRATION OF ANTIHUMAN HEMOLYSIN 



1. Secure 0.1 c.c. of inactivated antihuman rabbit amboceptor serum and dilute 

 1: 100 by adding 9.9 c.c. normal saline solution. 



2. To a series of six small test-tubes add increasing amounts of diluted ambo- 

 ceptor as follows: 0.1, 0.2, 0.4, 0.6, 0.8, and 1 c.c., add 0.1 c.c. of 40 per cent, com- 

 plement, 1 c.c. of 1 per cent, human corpuscle suspension, and sufficient saline solu- 

 tion to bring the total volume to 2 c.c. 



3. Incubate for one to two hours, shaking the tubes once or twice during this 

 time. 



(a) Are there any evidences of hemagglutination? 



(b) What constitutes the unit of hemolytic amboceptor? 



(c) Is an antihuman hemolytic system more delicate than an anti- 

 sheep system in complement-fixation work? 



(d) What are the advantages of using an antihuman hemolytic sys- 

 tem? 



EXPERIMENT 93. TECHNIC OF THE NOGUCHI MODIFICATION 



1. Secure five specimens of blood not over twenty-four hours old, including at 

 least one positive and one negative serum. 



2. Conduct the Noguchi reaction with each serum in the unheated or active 

 state, using an antigen of acetone-insoluble lipoids. (See page 450.) 



3. Conduct the reactions with the same antigen, using the serums after heating 

 to 56 C. for half an hour. 



(a) Record your results. Are they equal in both series? 



(b) What is meant by proteotropic reaction? 



(c) What effect has heat upon syphilis reagin? 



(d) Does an active serum react more delicately than an inactivated 

 one? 



(e) In performing this test with unheated serum what precautions 

 are to be observed? 



EXERCISE 42. WASSERMANN AND NOGUCHI REACTIONS 

 EXPERIMENT 94. COMPARISON OF METHODS 



1. Secure five serums and a cerebrospinal fluid. 



2. Conduct a Wassermann reaction with each after the second method. 



3. Conduct a Noguchi test with each serum inactivated and using an extract of 

 acetone-insoluble lipoids. 



(a) How do the results compare? 



(b) Which reactions are more easily read? 



(c) Discuss the relative delicacy and value of the Wassermann and 

 Noguchi reactions with both active and inactivated serum in the latter. 



