VENOM HEMOLYSIS 861 



EXERCISE 46. COMPLEMENT FIXATION IN THE DIFFERENTIATION OF 



PROTEINS 



EXPERIMENT 98. TITRATION OF IMMUNE SERUMS 



1. Secure 1 c.c. each of antihuman and antihorse serum. Inactivate both. 



2. Secure 0.1 c.c. each of fresh human and horse serum and dilute 1: 1000. 



3. Conduct an antigenic titration of each immune serum as described on page 495. 



(a) Explain the mechanism of this reaction. 



(b) Are protein amboceptors specific? 



(c) Discuss the question of inhibition of hemolysis due to a precipitin 

 reaction. 



EXERCISE 47. COMPLEMENT FIXATION IN THE DIFFERENTIATION OF 



PROTEINS 



EXPERIMENT 99. TECHNIC OF THE FORENSIC BLOOD TEST 



1. Secure from the instructor two pieces of gauze stained respectively with human 

 and horse blood. These are to be numbered and their identity known only to in- 

 structor. 



2. Secure 1 c.c. each of antihuman and antihorse serum. 



3. Secure 0.1 c.c. of known human and horse serum for controls. 



4. Proceed with the test for identifying these bloods as described on page 494. 



(a) Discuss the specificity of this test. 



(b) Is this test more delicate than the precipitin reaction? 



(c) Which is more liable to error in technic? 



(d) Discuss the applicability of the complement-fixation technic to 

 the differentiation of proteins in general, as animal, vegetable, and bac- 

 terial proteins. 



EXERCISE 48. VENOM HEMOLYSIS 

 EXPERIMENT 100. VENOM HEMOLYSIS IN SYPHILIS 



1. Secure 1 c.c. of venom solution 1:1000. 



2. Secure four specimens of blood (for technic see page 386) from cases of late 

 secondary or tertiary syphilis and one specimen of normal blood. Also a known 

 normal blood for a control. 



3. Prepare the subdilutions of venom and conduct the tests after the technic 

 described on page 387. 



(a) Discuss the prevailing views regarding the mechanism of venom 

 hemolysis. 



(b) Discuss the value of the venom test in the diagnosis of syphilis. 



(c) Discuss the main points in the technic. 



