864 EXPERIMENTAL INFECTION AND IMMUNITY 



(c) How do you explain the presence of hemolysin in this immune 



serum? 



(d) Discuss the specificity of cytotoxins in general. 



(e) How may a hemolysin be removed from a nephrocytotoxic 



serum? 



(f) Have the cytotoxins any practical value in the treatment of dis- 

 ease? 



EXERCISE 53, MIOSTAGMIN REACTION 

 EXPERIMENT 105. TECHNIC OF THE MIOSTAGMIN REACTION 



1. Secure a portion of dry pulverized cancer tissue and prepare an antigen after 

 the technic described in the text. 



2. Titrate this antigen. 



3. Secure four specimens of serum: one from a known case of cancer; one from 

 a normal person, and two for diagnosis. 



4. Proceed with the reaction as described in the text. 



(a) Discuss the principles of this reaction. 



(b) Discuss the practical value of this reaction in the diagnosis of 

 cancer. 



EXERCISE 54. ANAPHYLAXIS 

 For experiments in anaphylaxis sensitize a series of animals as follows: 



(a) Give seven young guinea-pigs an intraperitoneal injection of 0.01 

 c.c. horse serum (1 c.c. of a 1 : 100 dilution). 



(b) Give three young guinea-pigs an intraperitoneal injection of 0.01 

 c.c. of human serum. 



(c) Give two young guinea-pigs an intraperitoneal injection of 0.1 

 c.c. egg-albumen (1 c.c. of 1 : 10 dilution). 



(d) Give two rabbits 1 c.c. of horse serum intravenously every three 

 days for three doses. 



(e) Give a dog 10 c.c. of horse serum siibcutaneously. 



EXPERIMENT 106. ANAPHYLAXIS IN THE GUINEA-PIG. SPECIFICITY 



OF ANAPHYLAXIS 

 1. Two weeks after the sensitizing injections proceed as follows: 



(a) Inject a guinea-pig sensitized to horse serum with 1 c.c. horse serum intra- 

 peritoneally. 



(b) Inject a guinea-pig sensitized to human serum with 0.1 c.c. of human serum 

 intravenously. 



(c) Inject a guinea-pig sensitized to egg-albumen with 1 c.c. of diluted (1:4) 

 albumin intraperitoneally. 



