866 EXPERIMENTAL INFECTION AND IMMUNITY 



2. After recording the normal blood-pressure tracing, inject 5 c.c. of homologous 

 serum (horse) into a jugular vein. 



3. Record the blood-pressure. 



4. Study the coagulation time of the blood. 



5. Autopsy. 



(a) Describe the blood-pressure changes. To what may they be 

 ascribed? 



(b) Is blood coagulation delayed? Give a reason for this change. 



(c) Do anatomic changes occur in anaphylaxis of the dog? 



EXERCISE 57. ANAPHYLAXIS (Continued) 

 EXPERIMENT 109. PASSIVE ANAPHYLAXIS 



1. Secure 1 c.c. of serum from a rabbit sensitized to horse serum five weeks previ- 

 ously and inject 0.5 c.c. intraperitoneally into each of two guinea-pigs. 



2. Twenty-four and forty-eight hours later inject both animals intravenously 

 with 0.2 c.c. of horse serum. 



(a) Are anaphylactic symptoms in evidence? 



(b) Discuss the nature of passive anaphylaxis. 



(c) If allowed to live, would these animals become sensitized to rab- 

 bit serum? 



EXERCISE 58. ANAPHYLAXIS (Continued) 

 EXPERIMENT 110. ANTI-ANAPHYLAXIS 



1. Eight days after sensitizing a guinea-pig with horse serum, inject 1 c.c. of 

 horse serum subcutaneously. 



2. Fifteen days after sensitizing a guinea-pig with horse serum, inject 2 c.c. of 

 serum into the rectum. 



3. Fifteen days after sensitizing a guinea-pig with horse serum, inject 0.0001 c.c. 

 serum subcutaneously every fifteen minutes for six doses. 



4. On the sixteenth day after sensitizing, inject a guinea-pig with 1 c.c. of horse 

 serum. If this animal develops anaphylactic shock, inject the other three guinea- 

 pigs in a similar manner. 



(a) Do anaphylactic symptoms develop? If not, why not? 



(b) Discuss the importance of quantitative factors in producing 

 anti-anaphylaxis. 



(c) Discuss the importance of anaphylaxis and anti-anaphylaxis 

 in serum therapy. 



(d) What method is generally employed in the effort to induce an 

 anti-anaphylactic state in serum therapy? 



(e) What drug has been found experimentally of value in ameliorat- 

 ing or preventing the pulmonary changes of anaphylaxis? 



