ANAPHYLAXIS CHEMOTHERAPY 867 



(f) Do anesthetics prevent anaphylaxis? 



(g) Under what conditions would you expect anaphylaxis in persons? 



EXERCISE 59, ANAPHYLAXIS (Continued) 

 EXPERIMENT 111. LOCAL ANAPHYLACTIC REACTIONS 



1. Shave the abdomen of the rabbit sensitized to horse serum five weeks after 

 the time of sensitization. 



2. Make two superficial abrasions over the upper portion of the abdomen; into 

 one rub horse serum, and in the other, normal salt solution. 



(a) Does a local reaction occur? If so, describe the lesion. 



3. Then inject 0.01 c.c. of serum subcutaneously. If acute anaphylactic death 

 does not follow, observe the animal during the next twenty-four hours. 



(a) Does a local Arthus reaction follow this injection of serum? If 

 so, describe the reaction. 



(b) Explain the mechanism of a local anaphylactic reaction. 



(c) Are the tuberculin, luetin, and mallein reactions anaphylactic? 

 Explain their mechanism and discuss their practical value in diagnosis. 



4. Inject 1 c.c. horse serum intravenously. If anaphylaxis occurs, record the 

 symptoms. Autopsy with particular attention to the heart. 



EXERCISE 60. CHEMOTHERAPY 

 EXPERIMENT 112. SALVARSAN 



1. Secure a white rat infected with Sp. recurrens (Sp. Obermayer) and one with T. 

 equiperdum. Also two normal rats. 



2. Examine a drop of blood from the tail of each infected rat and form a rough 

 estimate of the number of parasites per microscopic field. 



3. Inject both rats intravenously with 0.001 gm. salvarsan freshly prepared. 



4. Examine the blood at the end of two hours; after forty-eight, seventy-two, 

 and ninety-six hours. 



5. Inject a control rat intravenously with 0.01 gm. salvarsan and the second with 

 0.05 gm. 



(a) Do the control rats survive? 



(b) Are the infected rats sterilized? 



(c) What is meant by the dosis lethalis? By the dosis curativa 

 By the dosis tolerataf 



(d) Discuss organotropism and parasitotropism. 



(e) Discuss ''drug fastness." 



(f) Discuss the question of chemoreceptors. 



