METHODS OF INOCULATION 



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ized and ready for use. Two sterile graduated centrifuge tubes should 

 be on hand for collecting and measuring the spinal fluid. The apparatus 

 should be assembled and ready for injection, so that at the appointed 

 time the tubing may be attached to the needle and the inoculation given. 

 3. The patient should be placed on the left side, on the edge of a bed 

 or table. An assistant places the patient in such a manner as to arch 



FIG. 142. INTRASPINAL INJECTION BY GRAVITY. 



The line marks the crest of the ilium, and indicates the third lumbar interspace. 

 The needle has been inserted and cerebrospinal fluid withdrawn. Antiminingococcus 

 serum is being administered. The barrel of a 20 c.c. Record syringe is serving as a 

 funnel, and is attached to the needle by means of 18 inches of soft-rubber tubing 

 furnished with a metal tip. The needle, as shown, is reduced to about four times its 

 actual size. This method may be used for making the subdural injection of tetanus 

 antitoxin and salvarsanized serum. 



the back as much as possible. A second assistant takes blood-pressure 

 readings during the operation. 



4. Towels wet with bichlorid are arranged about the site of inocula- 

 tion. It is well for the operator to locate the site of injection by pal- 

 pating the spinous processes and selecting the widest interspace, which 

 is usually on a level with the crests of the ilia if the back is well arched. 

 The skin is then cleansed with soap, water, and alcohol, and bichlorid 

 solution or a coat of iodin applied. Some degree of local anesthesia may 

 be secured by injecting a small amount of a sterile 1 per cent, solution 

 of eucain. This is advisable in nervous adults. 



