NOGUCHI MODIFICATION OF THE WASSERMANN REACTION 935 



EXERCISE 39. WASSERMANN REACTION (Continued) 

 EXPERIMENT 91. WASSERMANN REACTION (THIRD METHOD) 



Conduct a Wassermann reaction with each of four specimens of serum after 

 the third method, using an extract of acetone-insoluble lipoids. One of these serums 

 should be a positive control and one a normal or negative control. 



What is the advantage of using a quantitative test? 



EXERCISE 40. WASSERMANN REACTION (Continued) 

 EXPERIMENT 92. WASSERMANN REACTION (FOURTH METHOD) 



Conduct a Wassermann reaction with a known positive, a known negative, 

 and two unknown serums after the fourth method, using an extract of aceton-insoluble 

 lipoids. 



What are the main advantages of this technic? 



EXERCISE 4*. NOGUCHI MODIFICATION OF THE WASSERMANN RE- 

 ACTION 



EXPERIMENT 93. TITRATION OF ANTIHUMAN HEMOLYSIN 



1. Secure 0.1 c.c. of inactivated antihuman rabbit amboceptor serum and dilute 

 1:100 by adding 9.9 c.c. normal saline solution. 



2. To a series of six small test-tubes add increasing amounts of diluted ambo- 

 ceptor as follows: 0.1, 0.2, 0.4, 0.6, 0.8, and 1 c.c., add 0.1 c.c. of 40 per cent, com- 

 plement, 1 c.c. of 1 per cent, human corpuscle suspension, and sufficient saline solu- 

 tion to bring the total volume to 2 c.c. 



3. Incubate for one to two hours, shaking the tubes once or twice during this 

 time. 



(a) Are there any evidences of hemagglutination? 



(b) What constitutes the unit of hemolytic amboceptor? 



(c) Is an antihuman hemolytic system more delicate than an anti- 

 sheep system in complement-fixation work? 



(d) What are the advantages of using an antihuman hemolytic sys- 

 tem? 



EXPERIMENT 94. TECHNIC OF THE NOGUCHI MODIFICATION 



1. Secure five specimens of blood not over twenty-four hours old, including at 

 least one positive and one negative serum. 



2. Conduct the Noguchi reaction with each serum in the unheated or active 

 state, using an antigen of acetone-insoluble lipoids. (See page 475.) 



3. Conduct the reactions with the same antigen, using the serums after heating 

 to 56 C. for half an hour. 



(a) Record your results. Are they equal in both series? 



(b) What is meant by proteotropic reaction? 



(c) What effect has heat upon syphilis reagin? 



