APPENDIX. rfy 



5. Hsematoxylin, Solution of. 



a. Prepare a saturated solution of crystallized calcic 

 chloride in 70 per cent, alcohol ; then add alum to 

 saturation. 



b. Prepare a saturated solution of alum in 70 per cent, 

 alcohol. Add I volume of a to 8 of b. 



c. To the mixture of a and b add a few drops of a 

 saturated solution of pure hsematoxylin in absolute 

 alcohol. Filter. 



6. Iodine, Solution of. 



Prepare a saturated solution of potassic iodide in dis- 

 tilled water; saturate this solution with iodine. Filter. 

 Dilute to a brown sherry colour. 



7. Magenta, Solution of. 



Dissolve I decigr. of crystallized magenta (roseine) in 

 160 cubic centimetres of distilled water: add I cub. cent, 

 of absolute alcohol. Keep in a well-closed bottle. 



8. Mayer's Solution. 

 See note p. 8. 



9. Mullens Solution. 



Bichromate of potash 25 grammes. 



Sodic sulphate 10 grammes. 



Distilled water I litre. 



10. Osmic Acid, Solution of. 



Best bought ready made in the form of I per cent, solu- 

 tion. 



11. Paraffin. 



Melt together one part of solid paraffin (paraffin candles 

 will do), one part of paraffin oil and one part of pig's 

 lard. A mixture in the above proportions gives, when it 

 has cooled, a mass of the most generally useful con- 

 sistency. 



