24 



HARRY WARREN ANDERSON 



died during the course of the experiment. Oidium albicans culture was secured 

 from St. Thomas' Hospital, London, through the kindness of Dr. H. D. Singer 

 of Kankakee. It was isolated from the mouth of a child who was suffering 

 from thrush. The other named cultures were obtained from the "Centralstelte 

 fur Pilzkulturen," Amsterdam, Holland. Saccharomyces hominis Busse is sup- 

 posed to be a subculture of Busse's ('95) original organism. Guilliermond 

 states, however, that this organism is supposed to ferment glucose and not 

 liquefy gelatin, neither of which reactions agrees with the results given in 

 Table 3. Saccharomyces ellipsoideus is probably a composite species. The data 

 under the head of 'type' give merely the type of growth of young agar slants. 

 Under 'gelatin' is included a rough division into the 3 types outlined earlier. 

 The reactions with sugars are based, in each case, on a number of trials. On 

 the basis of the fermentation of sugars alone, the 20 species may be separated 

 into the following groups : 



Group 1. Those not fermenting sugars 



11.5, 9.1, 128, 215, 172, 138, 137, S. hominis, 

 141.2 



Group 2. Those fermenting all except lactose 158, 152, 229 M. Candida S. ellipsoideus 

 Group 3 Those fermenting glucose, maltose 



and levulose but not sucrose, 



galactose, or lactose 2.5, D. O. albicans, 147 



Group 4. Those fermenting glucose and 



levulose only 141.1, 170 



Group 5. Those fermenting glucose, sucrose, 



maltose and levulose, but not 



galactose or lactose 



It has been found, as mentioned, that there is some variation in the reac- 

 tions toward sugars. This is especially true of the members of the 3rd group; 

 sucrose and galactose are fermented at times by these organisms, and at other 

 times not. 



TABLE 4 

 CHANGE IN ACID REACTION BROUGHT ABOUT BY 20 CULTURES INCUBATED AT 35 C. FOR 6 DAYS 



* The results are given in terms of the number of cubic centimeters of n/10 NaOH 

 required to neutralize 100 c.c. of the culture solution, assuming the original reaction to be 

 exactly neutral. If there was a loss of acidity it is expressed by the negative sign. 



