42 HARRY WARREN ANDERSON 



Physiologic Properties. It does not ferment glucose, sucrose, levulose, 

 maltose, galactose, or raffinose. No decided change in acidity occurs in these 

 mediums. There is no change in litmus milk. 



The culture was isolated from human feces. (Culture 11.501; Type Speci- 

 men 3; Type Slide 11.5.) 



CRYPTOCOCCUS VERRUCOSUS SP. NOV. 



Morphology. In young liquid culture the cells are oblong, narrowly ellip- 

 tical or oblong-elongated; in old cultures elongated cells are common, with 

 several 'oil' globules in each cell. The size is 3 X 9 microns. Budding occurs 

 from shoulders, ends, or sides. No endospores are formed. (Plate 5, Figs. 1, 2.) 



Cultural Characters. On glucose agar slant there is at first an even, filiform, 

 glistening, white, smooth growth; later it becomes dull, brittle, verrucose, and 

 pulvinate. On carrot slant the growth is more profuse, with verrucose char- 

 acter more pronounced, and with chalky-white surface. There is a filiform 

 or nodose growth in gelatin stab, with no liquefaction. On sugar mediums 

 and beerwort, after 2 days, a few small, white patches appear on the surface, 

 later becoming larger, dry, and very firm; at first they remain separate, but 

 later coalesce. Giant colonies may be seen in Plate 6, Figure 10, and in Plate 

 8, Figure 14. 



Physiologic Properties. It does not ferment glucose, levulose, sucrose, 

 maltose, galactose, lactose, or raffinose. No decided change in acidity occurs 

 in these sugars. Litmus milk becomes very slightly alkaline after several weeks. 



The culture was isolated from human feces. (Culture 172.1; Type Specimen 

 2; Type Slide 172.) 



The dry brittle character of the colonies on solid mediums, the formation 

 of the isolated, white patches on all liquid mediums, and the peculiar type of 

 cells, clearly distinguishes this yeast from any other studied. 



CRYPTfJcOCCUS AGGREGATUS SP. NOV. 



Morphology. In both young and old cultures the cells are mostly globular 

 or slightly oval. No elongated cells are formed. Budding occurs from any 

 point on the cell, usually several buds arise from each cell; in old cultures 

 buds are commonly formed in large numbers about a single enlarged cell. 

 The size is 3.5 microns. (Plate 4, Figs. 1, 2.) 



Cultural Characters. On glucose agar slant the growth is filiform, convex, 

 glistening, smooth, chalk-white, and firm. In old cultures the surface remains 

 smooth, with even edges and no darkening in color. Filiform, later nodose 

 growth occurs in gelatin stab, with no liquefaction. No pellicle or ring is 

 formed in beerwort or liquid sugar mediums. The surface of the giant colonies 

 on glucose agar plates remains remarkably smooth, only dim, concentric lines 

 appearing. (Plate 6, Fig. 12; Plate 8, Fig. 19.) 



Physiologic Properties. There is no fermentation in glucose, sucrose, levulose, 

 maltose, galactose, lactose, or raffinose yeast water. No decided change in 

 acidity occurs in these sugar mediums. Litmus milk becomes very slightly 

 alkaline after 3 weeks. 



The culture was isolated from human feces (Culture 215.103; Type Speci- 

 men 3; Type Slide 215). 



Two other cultures, 215.101 and 215.102, isolated from the same person were 

 compared with the foregoing species and found to be identical. The isola- 

 tions were made from the same sample of feces but from different colonies. 



