44 HARRY WARREN ANDERSON 



hours on all liquid sugar mediums and on beerwort. There is a villous growth 

 along stab in gelatin. Giant colonies may be seen in Plate 7, Fig. 12, and in 

 Plate 8, Fig. 10. 



Physiologic Properties. Glucose and levulose ferment readily. There is no 

 change in litmus milk. Sugar mediums, with an original acidity of 1, become 

 less acid after 1 week. 



The culture was isolated from human feces. (Culture 141.101; Type Speci- 

 men 7; Type Slide 141.1.) 



MYCODERMA RUGOSA SP. NOV. 



Morphology. Cells in young cultures are elliptical, oblong, elongated, or 

 somewhat irregular; in old cultures the cells on the surface of the medium 

 are oblong, ovate, or elongated; beneath the surface very long, narrow cells 

 of hyphal character are produced by the elongation of the bud at the distal 

 end of another elongated cell. No septate mycelium is formed. Budding in 

 young cell occurs from end or shoulder. The size is 3 X 6.5 microns. (Plate 

 3, Figs. 8, 9.) 



Cultural Characters. On glucose agar slant the streak is white, dull, and 

 flat, but not spreading ; later the surface becomes glistening and decidedly rugose 

 and pitted. Bushy growths may extend downward into the agar at points along 

 the streak. There is a rapid villous development in gelatin stab cultures. A 

 heavy pellicle is formed in sugar mediums and beerwort. Giant colonies are 

 very distinctive. (Plate 7, Fig. 5.) 



Physiologic Characters. No sugars are fermented; there is.no change in 

 litmus milk. 



The culture was isolated from human feces. (Culture 128.1 ; Type Speci- 

 men 8; Type Slide 128.) 



This Mycoderma is not distinguishable from several other species, for 

 example, M. cerevisiae, as far as the morphologic and physiologic characters 

 enumerated are concerned. An examination of photographs of the giant colonies 

 of various Mycoderma species revealed the fact that none of these species 

 produce the peculiar rugose-pitted type formed by the foregoing species. The 

 production of such type of growth is not confined to giant colonies on glucose 

 agar, but is present on slants of glucose and beerwort agar. 



PARASACCHAROMYCES ASHFORDI SP. NOV. 



Morphology. In young cultures cells are round or slightly oval; in old cul- 

 tures cells are of many forms : oval, elongated, elliptical, round, or irregular ; 

 giant cells are common. Septate mycelium develops in gelatin hanging-drop 

 and in old cultures. Budding occurs from any point on the young cells, but 

 usually near the ends of articles in old cultures. The size is 4.5 X 5 microns. 

 (Plate 4, Figs. 4, 5; Plate 5, Figs". 11, 12.) 



Cultural Characters. On glucose agar the streak is filiform, raised, glisten- 

 ing, chalk-white and smooth; later the central portion may become rugose or 

 pitted; the edge of the streak may remain entire or may become decidedly 

 filamentous, due to the outward growing hyphal elements under the surface 

 of the medium (Plate 4, Fig. 5a). There is a growth in gelatin stab at first 

 filiform, later it develops scattered, bushy clusters of filaments. 1$ liquid 

 sugar mediums and beerwort a very evident ring formation occurs; no pellicle 

 is present. Giant colonies may be seen in Plate 7, Fig. 8, and in Plate 8, 

 Figs. 7, 8. 



