INTRODUCTION 



based upon what may be termed blood relationship or 

 descent, is uncertain. However, for practical purposes this 

 is of little moment, and they may be left as a division of 

 the Fungi, as indicated in the following scheme : 



Pterido-j Fern ?' Horse : 

 -I tails, and 



( Lycopods. 



phytes. 



2. B r y 



phytes. 



3. Thai 

 phytes. 



2. Animal 

 * dom. 



Ex. 1. To clean Slides and Cover-Slips. Before any satis- 

 factory microscopic work can be done, it is necessary to clean 

 thoroughly all glass slides and cover-slips or cover-glasses. 



(1) Place them in a hot solution of caustic soda (caustic soda 

 about 10 grams, water 100 c.c.) for an hour. 



(2) Take them out and wash under water-tap : wipe and dry 

 them. 



(3) Place them, one by one, in a beaker containing strong 

 nitric acid, and leave them for one or two hours. 



(4) Take them out, wash under tap, wipe and dry with a 

 clean handkerchief. 



(5) Store them in a beaker or specimen jar containing 

 absolute alcohol until wanted. When required for use, take out 

 and wipe clean. 



Ex. 2. Heat a platinum wire loop (Fig. 3) in a Bunsen flame ; 

 dip it just below the surface of an infusion of hay which has been 

 prepared in the manner described above and left in a warm room 



