AMMONIACAL FERMENTATION OF URINE 221 



Urobacillus Miquelii, Beijk. A motile bacillus 3 to 4 /a long, 

 i p broad, sometimes in pairs ; no spores are produced. It was 

 isolated from soil and grows in ordinary media. It is frequent 

 in soil and has little urea fermenting power. 



Gelatine. The colonies are yellowish-white or sometimes pale 

 rose, of irregular branched outline, closely resembling those of 

 B. Zopfii. After growing for a time on gelatine it loses its 

 power of hydrolysing urea, but becomes more energetic in 

 liquefying gelatine. 



Urobacillus Leubei, Beijk. A motile red-shaped organism 

 isolated from soil. It is 3 to 5 /a long, 1.5 /a. thick, and forms 

 oval spores, .8 to i ^ long : some of the spore-bearing cells are 

 occasionally Clostridium-\\\it. It grows on ordinary gelatine 

 media. 



Gelatine. The spore-bearing surface colonies are 2 to 3 p in 

 diameter, dull, yellow and thin. The colonies of organisms 

 which have not produced spores are brighter and more trans- 

 parent. The gelatine is not liquefied even in old cultures. On 

 ammonium carbonate gelatine the colonies are much larger and 

 closely resemble those of U. Pasteurii; the latter, however, does 

 not grow on ordinary beef gelatine, so that doubtful colonies 

 may be distinguished by transference to this medium. 



Planosarcina urese, Beijk. A motile sarcina found in garden 

 soil. It occurs in the form of packets of 4 to 8 or more cells, each 

 cell about .7 to 1.2 ^ in diameter. It produces round spores 

 .6 & in diameter. The power of fermenting urea is not so 

 marked as that of the other coccus forms. It grows on ordinary 

 beef gelatine. 



Gelatine. The colonies are flat, non-liquefying, pasty and 

 yellowish. 



Urobacillus Pasteurii, Miq., is an aerobic rod - shaped 

 organism, 2 to 5 //< long and i to i J ft broad, sometimes in pairs 

 or longer chains. It produces spores usually near the end of 

 the cells and not infrequently appears as a Clostridium form. 

 It is motile and is Gram positive. The optimum temperature 



