326 MILK AND DISEASE 



water alternately until no more colour washes out when placed 

 in the latter. 



(g) After decolourization stain for one minute with Loffler's 

 methylene blue and wash well : dry and mount in Canada 

 balsam. 



(ti) Examine with J and -^ in. objective : the acid-fast organisms 

 and spores of certain species which may possibly be present 

 appear red, other bacteria, pus and mucus are blue. 



Methods of checking or stamping out bovine tuber- 

 culosis. It is outside our province to discuss the 

 question of the methods to be adopted to deal with 

 human tuberculosis ; the farmer, however, should en- 

 deavour to stamp out the disease from his herds and 

 make an effort to prevent tuberculous milk from being 

 supplied to his patrons. 



(i) All animals suffering from disease of the udder, and 

 " wasters " in an advanced state of disease, should be 

 destroyed immediately in order to prevent them from 

 infecting the healthy stock. 



(ii) Much good can be done by isolation and sub- 

 sequent elimination of all animals which give evidence 

 of tuberculosis when tested with " tuberculin " : the test 

 should be applied at intervals for some considerable 

 period. 



Two kinds of tuberculin are in use, namely : ( I ) the 

 old, a filtered extract from a glycerine veal-broth culture 

 of Bs. tuberculosis ; (2) the new, a centrifuged extract of 

 a dried and thoroughly pulverized culture. 



When injected in very small quantities into the blood 

 of an animal suffering from tuberculosis, even in a 

 clinically unrecognizable form, there is a very marked 

 rise of 1.5 to 2.5 C. in twelve to fifteen hours. 



