APPENDIX 



A PAPER by Mr. W. A. Murrill,* on Tsuga Canadensis, has 

 reached us too late to incorporate his results in the body of 

 the text. 



The time which elapses between pollination and fertiliza- 

 tion is quite different from that we have given for Pinus. Two 

 weeks after pollination the archegonium initials become evi- 

 dent, one week later the neck of the archegonium is formed, 

 two weeks later the ventral canal cell is cut off, and five days 

 later fertilization occurs. As contrasted with Pinus, this period 

 of less than six weeks between pollination and fertilization is 

 remarkably short. 



The neck of the archegonium exhibits considerable variation, 

 consisting of one to four cells. The ordinary number is two, 

 and the wall between them is usually oblique, although it may 

 vary in direction from transverse to longitudinal. 



The author was not able to discover any passage of nu- 

 clei from the jacket cells to the central cell through wall 

 pores. 



The two male cells are unequal in size, the diameter of 

 the functional one being twice that of the other. It would 

 be interesting to know whether this results from an unequal 

 division of the body cell, as in Taxus, or from unequal 

 growth. 



The nuclei of both male cell and egg pass into the resting 

 stage before fusion, an observation directly at variance with 

 the reported behavior of these nuclei in Pinus. 



* The Development of the Archegonium and Fertilization in the Hemlock 

 Spruce (Tsuga Canadensis). Ann. Bot. 14: 583-607. pis. 31-32. December, 

 1900. 



187 



