SERUM DISEASE. 395 



with serum from tuberculous patients and then, 

 twenty-four hours later, injecting tuberculin or 

 tubercle bacillus emulsion. Bail passively sensi- 

 tized guinea-pigs by the injection of tuberculous 

 tissues and in this way obtained anaphylactic 

 symptoms by injecting tuberculin after twenty- 

 four hours. Helmholtz produced passive sensiti- 

 zation against cutaneous reaction by the injection 

 of serum from tuberculous guinea-pigs into nor- 

 mal guinea-pigs so that after twenty-four hours, 

 they gave a positive v. Pirquet test. 



In contrast to the results of Yamanouchi and 

 Bail, other investigators have succeeded in passive 

 sensitization of guinea-pigs by injection of serum 

 from tuberculous animals either only occasionally 

 or not at all. Production of anaphylaxis by active 

 sensitization with tuberculin is possible only after 

 repeated large doses. It would seem, therefore, 

 that as in the case of other bacteria, typhoid, dys- 

 entery, etc., the anaphylaxis is against the proteins 

 of the tubercle bacillus rather than the toxin pro- 

 duced by it. 



The untoward symptoms following the injec- serum DI- 

 tion of curative serums has been the subject of 

 study by many investigators, particularly v. Pir- 

 quet and Schick, Eosenau and Anderson, and 

 Weaver. 



The reaction following a primary injection of 

 serum appears after a period of time varying from 

 a few minutes to several weeks. There may be 

 slight redness and itching at the inoculation site, 

 and swelling of the adjacent lymph glands. The 

 prominent symptoms are fever, skin eruptions, 

 edema and joint pains. Slight albuminuria and 

 leukopenia have been noted. 



