PASTEUR TREATMENT. 707 



The table on page 708, taken from Marx, illus- 

 trates a "light" and an "intensive" treatment. 



This scheme is variously modified in different 

 institutes, especially in the direction of a more 

 rapid progression to virulent material. 



Other methods of attenuation are also used, as other 

 the following: Heating emulsions of fresh virus At e t*nuat f i<m. 

 at 58 C. for different lengths of time, or at dif- 

 ferent temperatures (80 to 30 C.) for ten min- 

 utes (Babes-Puscari) ; digestion of virus with nat- 

 ural or artificial gastric juice (Tizzoni and Cen- 

 tanni) ; the use of fresh but very dilute virus 

 (Hogyes). Ferran, in Barcelona, inoculates man 

 with the fresh unaltered virus fixe, and in nearly 

 2,000 cases but two cases of hydrophobia devel- 

 oped. This indicates clearly the low infectious- 

 ness of virus fixe for man. 



The tendency at present is toward the use of 

 fresh rabic virus for the prophylactic treatment of 

 hydrophobia. This is the method of Hogyes, and 

 also of Ferran. Hogyes' first injection consists of 

 3 c.c. of a 1 to 10,000 or 1 to 8,000 dilution of 

 the fresh rabic cord, and gradually within the next 

 fourteen days the concentration is increased until 

 1 c.c. of a dilution of 1 to 100 is given. 



In order to obtain a basis of comparison for 

 the different methods of treatment Harvey and 

 McKendrick have proposed an arbitrary unit of 

 standardization for rabic virus. For this purpose 

 they agreed to consider that 0.2 c.c. of a 1 per 

 cent, emulsion of the fresh virus fixe contains 

 1,000 units. From this it follows that 0.2 c.c. 

 of a 1 to 1,000 emulsion would contain 100 units, 

 and 0.2 c.c. of a 1 to 1,000 dilution, 10 units. 



