PASTEUR TREATMENT. 709 



a 1 in 200 emulsion. (3) In the same way the 

 M. I. D. (minimum infective dose) for an emul- 

 sion of three-day cord is 0.2 c.c. of a 1 in 200 

 emulsion. (4) The M. I. D. of two-day cord is 

 not greater than 0.2 c.c. of a 1 in 1,000 emulsion 

 and probably not less than 0.2 c.c. of a 1 in 2,000 

 emulsion. (5) The M. I. D. of one-day cord is 

 not greater than 0.2 c.c. of a 1 in 4,000 emulsion 

 and almost certainly not less than 0.2 c.c of a 

 1 in 8,000 emulsion (the lower accepted limit of 

 fresh material). (6) Fresh material is infective 

 (M. I. D.) in a dose of 0.2 c. c. of a 1 in 8,000 

 dilution and may be so in considerably higher 

 dilutions even up to 1 in 40,000, but with such 

 high dilutions the experimental errors become so 

 great as to preclude any more exact fixation of 

 the M. I. D." 



Although these results are not mathematically 

 exactj it is probable that they may be used as a 

 working basis, and from them it is possible to 

 calculate the number of units in a given amount 

 of rabic cord dried for different periods. Harvey 

 and McKendrick estimate that in both the Pas- 

 teurian method and that of Hogyes little more 

 than 25,000 units are administered during the 

 course of treatment. 



It seems unnecessary at this date to quote sta- 

 tistics to show the value of the Pasteur treatment. 

 Observations indicate that immunity is not fully 

 established until about fourteen days after the 

 completion of the treatment, and in a certain num- 

 ber of cases the disease develops before this time 

 has passed. The number of deaths after this pe- 

 riod is exceedingly small and has grown less with 



