APPENDIX 



ANALYTICAL TABLES 



(OUTLINE OF METHOD FOB DETECTION OF VARIOUS SUBSTANCES IN 



A MIXTURE.) 



The following Physical Properties should be noted : 



I. Appearance. 



A. Powder. Dust some on to a slide and examine under the 



microscope for starch grains and crystals. Dissolve some in 

 a suitable solvent. 



B. Solution. 



1. Opaque may be due to : 



(a) suspended fat globules -clear up with ether ; 



(b) certain inorganic salts clear up with mineral acid ; 



(c) certain proteins. 



2. Opalescent may be due to : 



(a) glycogen or starch iodine reaction ; 



(b) certain proteins. 



3. Deeply coloured suspect blood. 



II. Reaction. 



A. Acid may be due to : 



If due to free acid, ascertain whether this be 



1. a mineral acid or) i /-. i_ , 



2. an organic acid } a PP^ ^unzberg s test. 



If due to an organic acid, apply Uffelmann's test for lactic acid. 

 B. Alkaline test for carbonic acid (effervescence with mineral acid), 



ammonia (smell, etc.), caustic alkali. 



The following Chemical Tests should now be applied to suitable 

 quantities of the solution. 



I. For Carbohydrates. 



1. Apply Trommer's test. 



A. Positive indicates monosaccharides, lactose, or maltose. 



B. Negative, but complete solution of cupric hydrate obtained 



on adding caustic alkali, indicates cane sugar. Confirm 

 for this by boiling some of the solution with a mineral 

 acid for a minute or so, and applying Trommer's test 

 341 



